how to check organ(tissue)-specific expression profile of a gene - (May/15/2008 )

hello,
a basic question, I am doing westernblot of myocardin and I need to choose the positive and negative control.
so how to know its expression level at different organs or tissues so as to choose negative and positive control.
Thanks a lot!

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I find the quickest way to see where a gene is expressed is to go to www.jax.org. Go to 'mouse genome informatics'. Type in the gene name and you'll find heaps of info Including where your gene of interest is expressed. So having a quick look, heart would be a good first choice for your positive control

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Thank you so much. But I am actually detecting myocardin level of rat neonatal heart, and need to choose positive and negative organs or tissues. the website you suggest is a a good reference, but the information (particularly, on the expression at different organs or tissues) is limited.

Anyone knows if this can be solved with pubmed?

Thanks!

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[/quote]

Thank you so much. But I am actually detecting myocardin level of rat neonatal heart, and need to choose positive and negative organs or tissues. the website you suggest is a a good reference, but the information (particularly, on the expression at different organs or tissues) is limited.

Anyone knows if this can be solved with pubmed?

Thanks!
[/quote]

Well of course pubmed would solve it, but I was giving you a quick ref check to use. Wouldn't heart tissue be your positive and something like thymus be a good negative? Of course you'll get more information from pubmed, you'll just have to do a lot more reading

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