Compare the expression of two target genes - (Dec/21/2008 )
Hi,
I would like to compare the expression of two target genes in a tissue and I do not know wether this is feasible. Here is my problem:
I did real time PCR on inflamed human tissue looking for two target genes. Both were normalized to GAPDH using the deltadelta CT method.
Target 1 is a marker for the degree of inflammation within the tissue which is an important information for interpreting the data on target 2. Is there any possibility to correlate the relative expression of target 1 with the relative expression of target 2 in a statistically correct manner allowing me to build a target1/target2 ratio?
Thank you very much for helping me!
I would like to compare the expression of two target genes in a tissue and I do not know wether this is feasible. Here is my problem:
I did real time PCR on inflamed human tissue looking for two target genes. Both were normalized to GAPDH using the deltadelta CT method.
Target 1 is a marker for the degree of inflammation within the tissue which is an important information for interpreting the data on target 2. Is there any possibility to correlate the relative expression of target 1 with the relative expression of target 2 in a statistically correct manner allowing me to build a target1/target2 ratio?
Thank you very much for helping me!
I compare expression levels between genes, but I am wary of performing statistical tests on them. I am just not that confident in calculating the propagation of error. If you are using Sybr Green I chemistry, take into account amplicon size and efficiency.
We need a statistician on this one, but technically after normalizing to your housekeeping, what you get is the "nett" differential expression of both genes. Then you can compare and correlate these two genes. For eg, if your target 1 increases in fold change and target 2 increases as well, both after normalization, then it is ok to say that target 1 increase positively correlates with target2 increase, and vice versa, if it that's what you mean.
Chris
Something like REST2008 analysis is suitable for this, isn´t it?