Normal PCR for looking expression - (Oct/06/2008 )
Can we use normal PCR for looking expression ,if i use human cDNA as template and design primers in coding region(exon) ?
-sonica-
Nowadays qPCR is best sensitve AND acurate choice. Normal RT-PCT still can be used for semiquantification if cDNA-specific primer is designed.
-shRNAmir-