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Normal PCR for looking expression - (Oct/06/2008 )

Can we use normal PCR for looking expression ,if i use human cDNA as template and design primers in coding region(exon) ?

-sonica-

Nowadays qPCR is best sensitve AND acurate choice. Normal RT-PCT still can be used for semiquantification if cDNA-specific primer is designed.

-shRNAmir-