gene expression analysis - (Sep/30/2008 )
hello,
i am starting to run rt-pcr experiments to look at differences in levels of gene expression. i was wondering if anyone knows of a good resource to help with the data analysis. for instance, how to compare Ct values/normalize to housekeeping genes, etc. an introduction on the subject would be awesome.
thanks.
-medstudent1-
QUOTE (medstudent1 @ Sep 30 2008, 04:02 PM)
hello,
i am starting to run rt-pcr experiments to look at differences in levels of gene expression. i was wondering if anyone knows of a good resource to help with the data analysis. for instance, how to compare Ct values/normalize to housekeeping genes, etc. an introduction on the subject would be awesome.
thanks.
i am starting to run rt-pcr experiments to look at differences in levels of gene expression. i was wondering if anyone knows of a good resource to help with the data analysis. for instance, how to compare Ct values/normalize to housekeeping genes, etc. an introduction on the subject would be awesome.
thanks.
try here: http://pathmicro.med.sc.edu/pcr/realtime-home.htm
-lorvvv-