Surface expression - (Apr/09/2008 )
Whats the concept of biotynlation.
Does simple western blotting can not confirm the expression of protein.
-samita-
biotinylation allows you to amplify the signal from your detection system.
avidin (and streptavidin) has multiple (5, i think) binding sites for biotin.
if your antibody (usually the secondary) is biotinylated then you can bind (strept)avidin to one. then 2 or 3 biotinylated enzyme molecules (ap or hrp) can be bound to each antibody/(strept)avidin complex.
in this way you will have 2 or 3 enzyme molecules where you would normally have only one.
-mdfenko-
Hallo i am nut sure completly, biotinylation serve not just for evidence for protein expresion as western blot does, but can by used for expresion quantification
have a nice day
-baxapoptoaia-