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Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (Jan/19/2008 )

hi
I want to expres a protein of Serratia marcescens, and use the specific Shine-Dalgarno sequence. I find there is a stop codon at the S-D sequence upstream. Is it effect the protein expression? Thanks

-weetou-

QUOTE (weetou @ Jan 20 2008, 05:03 PM)
hi
I want to expres a protein of Serratia marcescens, and use the specific Shine-Dalgarno sequence. I find there is a stop codon at the S-D sequence upstream. Is it effect the protein expression? Thanks

Unless the stop is after the initiation ATG codon, it won't have any effect.

-swanny-