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Western blotting Related Discussions
  1. Western Blot - Any one ever use serum as a primary antibody? (reply: 2)
  2. secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
  3. Protein Loading and Western Blot - Western Blot (reply: 5)
  4. Unidentified problem with low concentrated samples - Variable limit of detection of my western (reply: 9)
  5. Western Blot: PSMAD 1/5/8 low signal! HELPPPPP! - HELP! (reply: 1)
  6. Strange aspect of bands in Western Blot - (reply: 2)
  7. western blot: why are proteins not separated? - (reply: 4)
  8. Reason of Cross-reactions in Western blot? - without specific band (reply: 5)
  9. High background in western blot - (reply: 4)
  10. optimal conditions for wet transfer in western blotting - (reply: 3)
  11. western blot, I have a signal without primary antibody - (reply: 6)
  12. WB vs Immunostaining - (reply: 1)
  13. bubbles and overheating of western blot - (reply: 2)
  14. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
  15. WB of beta amyloid - a simple method for WB to visualize b-amyloid? (reply: 3)
  16. Monoclonal Ab Western and ELISA issue - (reply: 2)
  17. Western blot control - Suggestions? (reply: 3)
  18. western blot from heart lysates - membrane extractions too (reply: 2)
  19. Western: bands spreading out? - (reply: 1)
  20. protease inhibitor, TRI reagent and western - (reply: 2)
  21. wobbly bands in WB - (reply: 3)
  22. wobbly bands in western blotting - (reply: 5)
  23. Boiling a Western Blot? - Does it work? (reply: 3)
  24. WESTERN TRANSFER PROBLEM - (reply: 6)
  25. smeared proteins western blot - (reply: 5)
  26. From Western To ELISA - HELP!? - Are my parameters acceptable for use in ELISA? (reply: 4)
  27. How to make gradient gel for western blot ? - (reply: 8)
  28. Substrate for AP conjugated secondary antibody - is it the same as Western blot? by NBT and BCIP? (reply: 2)
  29. daub on Westernblot, but not on Coomassie? - (reply: 1)
  30. Experimental design question for Western blot - (reply: 3)
  31. ELISA vs. Western Blotting - Information on repeatability and resolution of these 2 techs? (reply: 7)
  32. How to release antibodies from beads after IP? - in order to run ELISA after IP, not Western Blot. (reply: 7)
  33. western blot--prob - (reply: 2)
  34. beta actin vs GAPDH as loading controls for Western blotting - (reply: 1)
  35. amount of protein to load for a western? - (reply: 2)
  36. Western blotting with blood serum samples - (reply: 2)
  37. Western Crisis: Sudden Disappearance of a Signal - (reply: 2)
  38. My first western....advice please - (reply: 3)
  39. Cloudy Western - (reply: 4)
  40. Non-denaturing western blot - I need a transfer protocol for non-denaturing gel (reply: 1)
  41. Antibody reuse for Western blot - (reply: 7)
  42. Western Blotting help - (reply: 1)
  43. Preparing mouse brain lysates for western blotting - (reply: 2)
  44. Cytochrome C detection by Western Blot - a typical question (reply: 2)
  45. A few questions on SDS, coomassie staining & western - (reply: 6)
  46. Nuclear fraction marker for Western Blots - (reply: 4)
  47. Loading control for Western blots - (reply: 1)
  48. No bands observed on anti-His western blot - (reply: 11)
  49. Western Blot Blocking - Blocking for 1hr vs overnight (reply: 8)
  50. How to reduce amount of antibodies used in WB - (reply: 4)
  51. Can we re-use antibody solutions just like Western Blot? - to save antibody (reply: 3)
  52. unexpected western blot band - western blot (reply: 1)
  53. Western Blot Double Stain? - (reply: 4)
  54. Western for a 500kda protein - some troubles, need help! - (reply: 1)
  55. Nuclear extract loading control in Western blot - (reply: 3)
  56. Western blot of the supernatant - (reply: 5)
  57. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)
  58. Supernatant for western - (reply: 3)
  59. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
  60. CN staining in Western blot? - (reply: 2)
  61. How to reuse antibody in Western blotting - Share your experience (reply: 8)
  62. What high molecular weight loading control for Western blotting? - (reply: 3)
  63. Western Blotting - BCIP/NBT visualization (reply: 5)
  64. problem with transfer during western blot - western blot /sds page (reply: 2)
  65. Yeast Western - (reply: 1)
  66. Which quantity of purified protein in WB? - (reply: 2)
  67. Which quantity of purified protein in WB? - (reply: 1)
  68. Collagen bands in Western Blot - (reply: 2)
  69. Western Blot with HPLC purified samples - non specific binding??? need help desperately (reply: 1)
  70. Western blotting troubleshoot - (reply: 1)
  71. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
  72. Inconsistent Western blotting results - (reply: 3)
  73. Western Blotting : Can longer incubations require more washing? - (reply: 2)
  74. western blot normalization - (reply: 1)
  75. Non specific bands on western blot. - (reply: 2)
  76. Vertical streaks on WB following IP - (reply: 3)
  77. How to increase expression of protein in yeast? - Western from yeast and liquid beta-galactosidase assay (reply: 1)
  78. DMSO in western lysis buffer - (reply: 1)
  79. Wiggly 5 kDa band Western blot - (reply: 1)
  80. non-specific bands in western blotting - (reply: 3)
  81. Large bands (207kDa) showing up in CoIP western - (reply: 1)
  82. integrin beta1 western - (reply: 1)
  83. serum and Akt phosphorylation - Do I have use serum-free media to check pAkt when western blotting? (reply: 4)
  84. combining the results from different gels in western blot - (reply: 3)
  85. transfer conditions in western blot - (reply: 7)
  86. PVY western - (reply: 1)
  87. Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
  88. western blot problem - no expression on stable transfection. (reply: 9)
  89. cytochrome c western help - (reply: 6)
  90. Urea and Western Blot - (reply: 4)
  91. WB antibody to be used in IHC - (reply: 7)
  92. mouse and rabbit antibodies in western blot - (reply: 2)
  93. Neutralizing Antibodies - on westerns and IF (reply: 1)
  94. High Molecular weight reference for Western. Help! - (reply: 7)
  95. western blot against Caspases - (reply: 1)
  96. Used western blot film-how to do stripping? - (reply: 1)
  97. western blot - (reply: 2)
  98. western blot--protein concetration - too dilute?? (reply: 3)
  99. Western Blot problem! - (reply: 2)
  100. how to see A beta by western blot - (reply: 6)
  101. Westerns with serum isolations - What's your protocol? - (reply: 2)
  102. Western Blot of Bacteria - Need some advice on performing a Western Blot from Bacteria (reply: 3)
  103. Western Blot Background - Having high backgrounds on western blotting due to antibody issues. (reply: 2)
  104. Western blotting for Histones - (reply: 1)
  105. Membrane Froze - Did this ruin my results? - Western Blot not working anymore?? (reply: 2)
  106. what is the equation for quantitative western blot - (reply: 3)
  107. SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 3)
  108. Cell lysis method for running cell lysate on Western blot - (reply: 4)
  109. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)
  110. How to choose the right buffer for my western blotting? - (reply: 2)
  111. Reuse WB - (reply: 3)
  112. Stripping for Western blots - What is the common procedure for stripping? (reply: 4)
  113. western blot -- running the gel - (reply: 6)
  114. western transfer efficiency.. with or without methanol / SDS? - any differences? for what kindof proteins? (reply: 1)
  115. western blotting transfer buffer - re-usage possible? (reply: 5)
  116. western blot current setting.. for 1 or 2 blots together? - (reply: 1)
  117. Wester blot non specific bands caused by secondary - (reply: 3)
  118. A basic Western blot question - (reply: 2)
  119. A big problem of my WB today - (reply: 10)
  120. Western Blot for weakly expressed protein - (reply: 3)
  121. Myosine Light chain WB - (reply: 4)
  122. why need to do protein assay before western blot? - (reply: 3)
  123. help optimize an western blot for 6kd protein - western blot tranfer problem (reply: 16)
  124. which internal control for my western blot? - (reply: 6)
  125. gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
  126. western blotting loading control - (reply: 4)
  127. Problems with Western blotting of tissue homogenates - (reply: 3)
  128. High background on westerns - (reply: 5)
  129. Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
  130. WB reverse image solution?! - white band on darck background (reply: 2)
  131. Fluorescent detection of western blots - (reply: 3)
  132. Western vs. ELISA - (reply: 5)
  133. GFP western - (reply: 3)
  134. Storing protein extracted from tissues for Western blot - (reply: 9)
  135. Amount of Cell Lysate for Western Blot - (reply: 2)
  136. different western results when using acetone prep vs crude protein - (reply: 3)
  137. Gap43 protein western Blot - (reply: 7)
  138. help, :-( Actin completely blank on western blot - (reply: 6)
  139. pipetting technique for western blot sampling - (reply: 3)
  140. Which p-Serine antibody is suitable for IP and WB - (reply: 1)
  141. Caspase-1 Western spontaneous activation - Need help (reply: 1)
  142. How can I load equal amounts of protein into each well during SDS-PAGE? - SDS-PAGE, WB, BCA assay, Actin (reply: 7)
  143. problem with western blot - (reply: 4)
  144. Collagen I and V western blot - Blue Native PAGE - Blue Native PAGE (reply: 7)
  145. Problems after stripping Western blot - (reply: 5)
  146. 3 bands on WB instead of 1 - (reply: 4)
  147. western and trizol - western and trizol (reply: 1)
  148. Real time PCR - RT-PCR vs Western blot (reply: 8)
  149. Western blotting for membrane bounded protein - (reply: 7)
  150. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  151. Storing Western blot membranes - What should I do after detection and stripping? (reply: 4)
  152. western blotting - for longer exposures (reply: 1)
  153. Trouble when extracting Myosin Light Chain for WB - (reply: 1)
  154. My antibody does not work for IP, but works well fpr western? - antibofy not working for IP (reply: 12)
  155. Western Blot conditions for high molecular weight proteins - (reply: 2)
  156. Sponge pads in semi-dry westernblotting? - to save Whatman paper... (reply: 3)
  157. western blotting - (reply: 10)
  158. WB anti-caveoline1 not reproducible - (reply: 2)
  159. Western for membrane bound protein - (reply: 11)
  160. Problems with HMW proteins and Western blot - (reply: 5)
  161. WB vs ELISA ? - (reply: 2)
  162. how to prepare blood sample for western blotting? - (reply: 3)
  163. detecting small membrane protein by western blot - (reply: 4)
  164. Nitrocellulose or PVDF membrane for western blot - which one???? (reply: 8)
  165. How to stain MW in nitrocellulose membran? - Western bloting (reply: 7)
  166. Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
  167. How to quantify Western blot results/bands? - with ImageJ? Scion? Other programs? (reply: 4)
  168. Western kit for human samples? - (reply: 1)
  169. Simple anti-His WB not working - (reply: 4)
  170. Western Blot/SDS-PAGE samples - boiled or not boiled? - (reply: 2)
  171. Is western quantitative? - (reply: 13)
  172. can detet anything with my western membrane - (reply: 8)
  173. Western blot with Adipose cells - (reply: 1)
  174. Detecting Transcription factors in western blot - Hard time detecting LEF-1(TFs) (reply: 2)
  175. western blots - survivin - detection of survivin in Panc1 cells (reply: 2)
  176. western background problem - (reply: 3)
  177. discordance between western blot and ELISA - (reply: 10)
  178. WESTERN BLOT : upper band at 45 kDa in blot of cytochrome c - (reply: 2)
  179. [western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (reply: 2)
  180. WB trouble - no signal of low kd protein - (reply: 5)
  181. Preparing cell lysate from cell culture for Western blot - (reply: 4)
  182. Confirming IP with WB - Can I use the same antibody for both IP and WB? (reply: 5)
  183. How to choose the ready gel for western blotting - (reply: 3)
  184. Special procedures for phosphorylated proteins? - During isolation and western blot (reply: 3)
  185. blinking results in western blot - (reply: 2)
  186. Western Blot of cells in Laemmil - (reply: 5)
  187. Please help me with the western blot problem - (reply: 2)
  188. Serial Dilution and Western Question - (reply: 5)
  189. Different endogenous protein detection result in western and IHC - (reply: 3)
  190. Western Blot: Re-use of membrane? - (reply: 4)
  191. WB trasfer problem - (reply: 11)
  192. extract proteins for WB - (reply: 4)
  193. Renin protein detection in Western blotting - (reply: 2)
  194. for western blot - (reply: 1)
  195. Westerns from 3D collagen gels - (reply: 1)
  196. Western Blotting problem - p21 detection in MCF7 lysates (reply: 2)
  197. Western problem: bubbles forming INSIDE PVDF membrane - (reply: 4)
  198. non-specific binding in western blotting - non-specific binding in western blotting (reply: 7)
  199. Western blot signal gone - (reply: 5)
  200. Yeast protein extract preparation for a quick western blot - (reply: 5)
  201. Control protein in culture medium for Western? - (reply: 3)
  202. which is more sensitive: IP or western blot? - Looking for transcription factor - would IP help? (reply: 5)
  203. streptavidin HRP for Western - Protocol needed - (reply: 1)
  204. Western Blot Hollow "Ghost" Bands with ECF - (reply: 1)
  205. Incomplete transfer in western blot - ..and frown turned into smiley (reply: 3)
  206. peptide expresion, but no western blot bands. - (reply: 2)
  207. western blot - (reply: 6)
  208. Very odd western bands - (reply: 4)
  209. Western blot liquids - what is really toxic? (reply: 3)
  210. western blot for phosphorylated proteins - (reply: 3)
  211. minimum amount of cells used for western blot - (reply: 5)
  212. cathepsin C detection in Western blot - (reply: 1)
  213. Cytoplasmic marker for western blotting - (reply: 4)
  214. western blot transfer debate - (reply: 5)
  215. COX-2 positve control for western blotting? - COX-2 positive control for western blotting? (reply: 3)
  216. westerns, protein concentr and RIPA buffer - (reply: 2)
  217. Western Blots with (250 kDa) High Molecular Weight Protein - (reply: 2)
  218. Oligodendrocyte's and tubulin antibody in Western Blot - (reply: 1)
  219. total protein assay before western blot - (reply: 1)
  220. western blot for nuclear and cytoplasm seperation sample - (reply: 3)
  221. Trouble finding 5kDa protein in western blot - (reply: 5)
  222. Why 2 bands for housekeeping? - western blot (reply: 2)
  223. western blot- some bands not seen - western blot (reply: 1)
  224. getting rid of nonspecific background in westerns - (reply: 3)
  225. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
  226. How to get rid of non-specific bands on Western blots. - (reply: 9)
  227. milk sample in western blot - (reply: 1)
  228. western blotting transfer onto membrane:why does current increase? - At constant voltage, resistance increases. The current also increases. (reply: 5)
  229. Multiple antibodies in Western - (reply: 5)
  230. WB - Wet - Markers not transferring? - (reply: 2)
  231. Western blot problems - Please need Help!!! (reply: 12)
  232. Western Blot of myosin heavy chain - (reply: 3)
  233. Multiple bands in WB - (reply: 2)
  234. WB: protein amount/ lane ? - What is the optimal protein amount per lane in WB ? (reply: 6)
  235. Western blotting and immunofluorescence. Results don't match. - This is madness... (reply: 7)
  236. Protein Extraction from cell media for western blot - (reply: 3)
  237. Western Blot for Higher molecular weight Proteins - (reply: 1)
  238. Western problem : no signal - could that be linked to pH? (reply: 2)
  239. blurry, smeared bands on my western blots - new to the world of the westerns (reply: 4)
  240. Western blot problem--- bar shape band - (reply: 5)
  241. Problem with western, no signal - AP works, ECL doesn't (reply: 9)
  242. c-Myc antibody for Western - (reply: 1)
  243. LAT western blot... 36 and 75 kDa? - (reply: 3)
  244. problem on western blot - (reply: 9)
  245. Transparent lines in Western Blotting - (reply: 1)
  246. Transfer during western blotting - (reply: 8)
  247. Special Western Blot Troubles - help neede (reply: 8)
  248. ELISA antibody-use for WB? - (reply: 6)
  249. Smear in WB - (reply: 4)
  250. Western blot: Circular bands - artefact? - (reply: 4)
  251. western blotting - (reply: 1)
  252. WB for secreted protein? - (reply: 5)
  253. membrane protein western blot - (reply: 3)
  254. Western Blot Troubles - (reply: 10)
  255. pH of western blotting buffers - pH of western blotting buffers (reply: 3)
  256. Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
  257. Incomplete transfer of proteins for western blot - (reply: 4)
  258. re-probing western blot with chemi-luminiscent substrate - (reply: 2)
  259. Western Blot Incubations - what solution is best for overnight incubations? (reply: 3)
  260. collagen western blotting - (reply: 1)
  261. Smad4 - double band on Western blot - (reply: 3)
  262. Fluorescence detection of Western blots - (reply: 3)
  263. Adrenergic receptor proteins (western blot problems) - (reply: 10)
  264. WB advice - (reply: 2)
  265. Western Blotting for Large Proteins - (reply: 2)
  266. Advice to improve westerns - looking for ideas to clean up my WB results (reply: 13)
  267. PBS vs TBS for western blots - Background problems (reply: 5)
  268. MW marker for 150 kDa protein in Western blot - (reply: 5)
  269. western block milk - milky colour disappears after 1 hour of blocking (reply: 12)
  270. Humid and semi-dry Western blot - (reply: 1)
  271. Western blot membrance blocking and storage - (reply: 4)
  272. Western blots - positive control - (reply: 2)
  273. western on VEGF... I see no bands at all... ned help - (reply: 4)
  274. loading for western blot - (reply: 7)
  275. High Background in western Blotting - (reply: 2)
  276. Native PAGE and WB - (reply: 1)
  277. Using Westerns to check crosslinking in ChIP - (reply: 2)
  278. western blotting for cytochrome c - (reply: 4)
  279. Photoshop your Western Blots - (reply: 4)
  280. WB quick question - (reply: 8)
  281. Western Blot with DRG? - (reply: 1)
  282. loading controls for western. which one to choose? - (reply: 5)
  283. western blot of phosphorylated proteins - (reply: 4)
  284. Difference bwtween BME and DTT in sample buffer for WB after IP - (reply: 10)
  285. Why has does my developed blot look smeared? (Western Blotting) - Western Blotting Analysis (reply: 3)
  286. subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
  287. Straight line selection - defining area for western blot bands - (reply: 3)
  288. STAT-3/phosphoSTAT-3 western blot - (reply: 6)
  289. densitometry of western blot bands - (reply: 2)
  290. Western blot sample storage - (reply: 2)
  291. Western blot or western blot? - (reply: 23)
  292. How to interpret a Western Blot? - (reply: 5)
  293. Insufficient transfer in Western blotting - (reply: 8)
  294. western blot protocol - (reply: 1)
  295. Western blottting - Santacruz antibodies - (reply: 10)
  296. adhesion molecules by westerns - (reply: 3)
  297. Beta-actin, double bands - Problems with my western blot (reply: 3)
  298. western with nuclear extract - does high salt/glycerol affect running (reply: 2)
  299. western blot transfer - what's your opinion? (reply: 6)
  300. reducing and non-reducing condition in westernblot - (reply: 4)
  301. Blokcing step of Western blotting - (reply: 5)
  302. Western Blot- - Bands appear only nc eon the film! (reply: 3)
  303. Quick western method with pvdf? - anyone tried it? (reply: 7)
  304. how to evaluate the siRNA silence efficiency? - western or rtPCR (reply: 6)
  305. Help NO SIGNAL WITH WESTERNS - (reply: 4)
  306. Storing a western blot over the weekend - (reply: 4)
  307. Help needed with western blot with human serum - (reply: 2)
  308. Knockdown detected by western but not RT-PCR - (reply: 3)
  309. scanner for quantification of western bands - (reply: 1)
  310. What is west western blotting? - Want to know the details and difference between western blot and west (reply: 2)
  311. increased/decreased expression in western blot - (reply: 2)
  312. Actin bands not equal on Western blot - (reply: 5)
  313. Western Blot membrane disintegration - (reply: 5)
  314. protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
  315. Western Blot with Plasma - (reply: 7)
  316. Western Transfer and Detection of small protein - (reply: 6)
  317. use of Pierce stripping buffer in WB - (reply: 3)
  318. anti-BRCA1 (ab-1) for WB - (reply: 8)
  319. Troubleshoot: Western; no bands appear in reduced lanes - (reply: 8)
  320. black dot blackground on western - (reply: 7)
  321. problem with western blot - (reply: 5)
  322. problem with western blot - (reply: 7)
  323. Western Blot total loaded protein amounts per lane - (reply: 4)
  324. Western Blot Troubleshooting - Marker fading and other issues (reply: 7)
  325. how larg should my tissue sample be for western blotting? - (reply: 1)
  326. Strange phenomenon with western blot - Double bands (reply: 2)
  327. Reasons for wrong protein size on Western blot and how to deal with - (reply: 8)
  328. problem in western blotting - (reply: 5)
  329. Western blotting with room temperature buffers - protocol says NOT to use cold buffers (reply: 6)
  330. western to detect ATM - (reply: 3)
  331. Technical question about western blot - (reply: 8)
  332. Western Blot Feedback - (reply: 1)
  333. Trouble with Western blot transfer buffer - (reply: 11)
  334. white bands in wb - (reply: 4)
  335. western questions - (reply: 3)
  336. Many negative bands in WB - (reply: 4)
  337. retina homogenization for western - (reply: 4)
  338. western vs elisa - (reply: 4)
  339. Ghost bands in Western Blots - (reply: 5)
  340. TRANSFER (WESTERN BLOT) - Transfer Tips (reply: 25)
  341. problem with western of Estrogen Receptor-alpha in MCF-7 - (reply: 2)
  342. Antibody from short peptides - Time saving, but do they work for both West and ELISAs (reply: 4)
  343. WB with cell culture medium - (reply: 2)
  344. Need help with WB/IP sensitivity issues - As in way too sensitive! (reply: 3)
  345. Memrane for Western Blot- which are the best? - (reply: 5)
  346. Urgent stuff about western blotting - (reply: 9)
  347. Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
  348. western blot problem - mailed by baatrix (reply: 7)
  349. Stripping membrane of Western blots - Introduction (reply: 10)
  350. Need help! Problems on Western blotting - (reply: 7)
  351. too much protein for Western? - (reply: 5)
  352. WB for comparing the level of certain protein in two different cell lines - (reply: 2)
  353. Problems to confirm 2-DE results by western blotting - (reply: 3)
  354. about western blot - Please help me (reply: 12)
  355. about western blot - Please help me (reply: 10)
  356. cell culture western blot - (reply: 15)
  357. What is the maximum amount of protein that could be loaded in WB? - (reply: 2)
  358. failure with first western blot - (reply: 8)
  359. G protein coupled receptor - western blot - (reply: 1)
  360. G protein coupled receptor - western blot - (reply: 3)
  361. using two primary antibodies for WB? - (reply: 3)
  362. Western Blot - unspecific antibody binding - (reply: 9)
  363. Western: wrong 2. Ab- R MY RESULTS USELESS?! - (reply: 5)
  364. reducing background in western... - ...by blocking the antibodies (reply: 2)
  365. stripping western blot - (reply: 3)
  366. My ChIP product Western is dirty - (reply: 7)
  367. western - cells treated w/ protein synethesis inhibitor - (reply: 1)
  368. Why milk is used in Western blotting? - (reply: 29)
  369. direct / indirect detection in WB - (reply: 3)
  370. Questions about phospho-protein level in western blotting - (reply: 5)
  371. Silver stain vs Western blot - (reply: 5)
  372. can we purify the protein from westernblot membrane? - (reply: 5)
  373. Using Western Antibodies for IHC - General rules- suggestions (reply: 3)
  374. Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
  375. WB and tissue samples - (reply: 2)
  376. Is Western blot really quantitative? - (reply: 8)
  377. why couldn't I see my protein on western blot after ChIP? - (reply: 3)
  378. protein preparation for Western Blot - (reply: 6)
  379. darker bands in WB - (reply: 6)
  380. Question about high salt TBST in WB - (reply: 1)
  381. No bands in WB - (reply: 2)
  382. Western Blot on an IEF separated recombinant protein - (reply: 5)
  383. HELP! Western blotting saga - (reply: 3)
  384. can u give ur opinion in my western blot results? - (reply: 15)
  385. protein ladder detection for western blotting - (reply: 2)
  386. Preparing blocking solution for Western Blotting - what milk power to use? - western blot (reply: 11)
  387. curved bands in my WB - (reply: 8)
  388. serum samples for western - (reply: 7)
  389. what is the function of the sodium azide in the buffer? - western blot (reply: 3)
  390. ABout the solution in Western blot and SDS? - (reply: 5)
  391. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
  392. Western blotting -smearing effect - (reply: 6)
  393. Can antibody recommended for ELISA or WB be used on IHC? - (reply: 5)
  394. high backgroud on western - (reply: 2)
  395. Role of sodium azide in block solution of Western blot - (reply: 4)
  396. Multiple bands on Western blot for an expressed protein - (reply: 7)
  397. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
  398. AT1 receptor expression and quantification (WB) - need advice (reply: 1)
  399. Selecting epitomes of antibodies to apply in Western Blotting - (reply: 1)
  400. Western blotting - How to interprete (reply: 2)
  401. Verify the protein spots found by 2D using Western blot - The expression level change are different (reply: 2)
  402. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
  403. why some antibodies need overnight incubation for Western blot - (reply: 4)
  404. Western blotting for ER - problems - (reply: 7)
  405. How to increase Western blot sensitivity? - (reply: 13)
  406. Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
  407. Western blotting signal problem - very weird and does not make any sense. (reply: 10)
  408. SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)
  409. diffuse bands on western blots - (reply: 5)
  410. western blot milk dots - (reply: 10)
  411. Western blot gel running problem - Gel Electrophoresis (reply: 7)
  412. calculations of western bands - which software is better? (reply: 3)
  413. Use of Urea instead of SDS to denature protein for Western? - (reply: 2)
  414. problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
  415. western blot loading control for secreted saliva proteins - (reply: 2)
  416. my protein is 18Kda but runs as a 25kda on WB - why ? - (reply: 13)
  417. housekeeping in western blot - housekeeping in western blot (reply: 2)
  418. How to increase transfer efficiency for Western blot - (reply: 9)
  419. Western blot transfer problem - (reply: 7)
  420. nuclei fractionation for western blotting - (reply: 1)
  421. big molecular weight for western blot for brain sample - (reply: 2)
  422. help me ! dying about my western blot - (reply: 14)
  423. HepG2 cells, ideal passage number for western blot - (reply: 1)
  424. Western Blotting detection Antibodies........... - Are anti-sheep and anti-goat HRP interchangable? (reply: 2)
  425. Nuclear Marker for Western Blotting - (reply: 3)
  426. Western Blot Analysis - Technique for Protein determination (reply: 6)
  427. frozen tissue prep for IHC and westerns - (reply: 1)
  428. "Ponceau S" distorting the bands in western blot? - (reply: 5)
  429. Bacterial antibodies as the controls for Western - (reply: 1)
  430. phosphorylated protein Western blotting - HELP!! (reply: 10)
  431. Western Blot smudge - (reply: 5)
  432. Immunofluorescence microscopy, Western Blot, ELISA, ... - (reply: 4)
  433. western blotting need help can i not strip if using same ab - (reply: 4)
  434. Okay to use different exposure times to quantitate a western? - (reply: 1)
  435. Unequal signal intensity across Western blot membrane - (reply: 4)
  436. How to strip PVDF membrane after imaging ? - For western blot (reply: 2)
  437. Problem in choosing internal contol for Western blot - not actin, tubulin, GAPDH and VDAC (reply: 11)
  438. how to prepare total cell lysate for Western blot? - (reply: 10)
  439. Western band distorted by 'bubbles' - (reply: 8)
  440. Who sells good primary antibodies for Westerns? - Who's your favourite supplier? Least favourite? (reply: 24)
  441. western blotting membranes - (reply: 6)
  442. One Antibody in Western Blot - (reply: 4)
  443. Western blot ECL quantification without previous Bradford/Lowry assay... - (reply: 3)
  444. Extra band in western blot - (reply: 1)
  445. Western blotting trouble shooting - Protein transfer (reply: 4)
  446. Harvest of cells from 96 well plates for Western blots - (reply: 8)
  447. ERK by Westerns - (reply: 8)
  448. quantification of WB bands- how to get similar values from different experiments - (reply: 4)
  449. how to do western with 500 kD protein? - (reply: 5)
  450. DsRed fusion protein not glowing - It is there by WB though (reply: 3)
  451. Blocking Western with Fab fragment - (reply: 3)
  452. Western blot bands fade out - (reply: 9)
  453. lysate for ELISA can be used for WESTERN? - (reply: 2)
  454. Problem with western blot with VEGF receptor 1 and 2 (ab2350 and ab2349) - (reply: 1)
  455. multiple bands in western blot - (reply: 5)
  456. Not getting actin bands equal on Western blot - (reply: 11)
  457. western blot after ChIP - necessary to revert crosslinking? (reply: 6)
  458. Western after Ip- no IP protein, but .... - (reply: 1)
  459. immunoprecipitation and western blotting assay by the same antibody - (reply: 4)
  460. Western Blot - membrane-bound protein boiling step - (reply: 1)
  461. Ponceau S as loading control on Westerns? - (reply: 3)
  462. What is Far Western Screening - (reply: 3)
  463. western blotting problem: b-actin is uneven - (reply: 6)
  464. Better resolution on SDS-PAGE then Western.... - (reply: 2)
  465. polyclonal antibody doesn't pick up my protein during western!? - (reply: 4)
  466. Western blotting without transfering protein to membrane - (reply: 4)
  467. Reverse Image with Western Blot - (reply: 1)
  468. Western AB unselective or posttranslational modifications? - (reply: 1)
  469. Tips and tricks for the perfect Western Blot - (reply: 23)
  470. no reference in the westerns blot (like actin) - are these results publishable? (reply: 7)
  471. Western Blot - fixation of proteins with glutaraldehyde? - (reply: 4)
  472. I have problem on Actin band - SDS-PAGE and Western blot (reply: 3)
  473. WB Loading control for large protein - (reply: 1)
  474. how to make neutrophil cell lysates for Western blot? - (reply: 3)
  475. terrible streaks on western bands - (reply: 7)
  476. Western Blot on an IEF separated recombinant protein - (reply: 6)
  477. DICER Western Blot - (reply: 5)
  478. milk in western blotting - (reply: 2)
  479. Western blot for two proteins: any alternative to stripping? - (reply: 11)
  480. Why two bands in my Western blotting - (reply: 1)
  481. Advice wanted on Western Blotting materials - (reply: 5)
  482. western blot ECL vs fluorescence - (reply: 2)
  483. Purified antibodies in Western. - How can I find out how much to use? (reply: 12)
  484. normalizing for loading and transfer error during western blot - (reply: 4)
  485. Cell lysing for western blot (Help!) - (reply: 2)
  486. Western Blotting substrate - Lumi-Light Western Blotting Substrate (reply: 4)
  487. Problem with pRb phosphospecific WB - Problem with pRb phosphospecific WB (reply: 3)
  488. Non specific bands on Western blot - (reply: 1)
  489. Western Blot of PKCalpha - (reply: 1)
  490. Problem of spontaneous MAPK detection from Western Bolt - (reply: 9)
  491. Cell Lysates for western - (reply: 4)
  492. Normalizing Western Blot to cell number without cell counting - (reply: 5)
  493. western blot quantification - band quantification (reply: 4)
  494. western blot looks strange - western blot looks very weird (reply: 5)
  495. Unspecific band in Western Blot - (reply: 11)
  496. western after BN-page - (reply: 6)
  497. Western Blotting band - (reply: 3)
  498. Protein yeast extraction for Western blot - (reply: 3)
  499. western protein ladder - (reply: 9)
  500. An annoying Western blotting problem - (reply: 3)
  501. protocol for P-P38 western please - (reply: 1)
  502. Western blooting interenal control problem - Protein and proteomic (reply: 3)
  503. Western blot bands look like "M" - (reply: 6)
  504. western blot - missing expected band - (reply: 1)
  505. western blot secondary antibody incubation time - will long incubation cause high background? (reply: 3)
  506. Washes for Western, PBS or TBST? - (reply: 12)
  507. Western blot bands look like 'upside down smiles' - (reply: 4)
  508. Two antibodies on one western Blot? - (reply: 4)
  509. PKC western blot - (reply: 1)
  510. Western blot problems... - Western blot: Bad blotting? (reply: 7)
  511. Western blotting problem - Protein and proteomic (reply: 6)
  512. Loss of signal during film exposure western blot - (reply: 1)
  513. western blotting of tissues - (reply: 1)
  514. many bands other than my protein on western... - please help me to explain this... (reply: 5)
  515. Favorite Western blot transfer apparatus - (reply: 2)
  516. membrane protein western blot - (reply: 2)
  517. Western/Signaling Studies - How to get rid of background phosphorylation? - Western/Signaling Studies - How to get rid of background phosphorylation? (reply: 3)
  518. westernblot problem! - membrane protein! (reply: 2)
  519. big black spot in the place of my protein on western... - (reply: 4)
  520. Question on stripping westerns... - (reply: 5)
  521. PVDF versus nitrocellulose - which is better for simple Western? - "normal" protein of 13kDa (reply: 4)
  522. How many cells to lyse for Western? - Decreasing signal in my Western Blot (reply: 2)
  523. western blot problem - antibodies can't detect the protein on the membrane (reply: 3)
  524. detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
  525. Liver tissue lysis for ER protein Western blotting - (reply: 2)
  526. western blot problems on getting signal - (reply: 5)
  527. 38kda protein appearing as smear on western - (reply: 5)
  528. Western Blotting - Old school - Looking for old protocol (reply: 3)
  529. Several questions about Western blotting of tissues - (reply: 2)
  530. Western Blots on media - (reply: 4)
  531. Getting my primary antibody for Western - (reply: 6)
  532. conditioned media for western - (reply: 1)
  533. Western Blotting protocol-specific questions - (reply: 3)
  534. Tween function in Western blot - (reply: 2)
  535. How to choose an antibody? - for western or other applications (reply: 1)
  536. Western Blot Hela Cos7 - Show nearly identical genes of two related organisms same expression (reply: 4)
  537. Blank smears on western blot - (reply: 7)
  538. Western blots - fuzzy below 100 kDa - (reply: 3)
  539. DNA in western blot - (reply: 4)
  540. strange bands on western blot - (reply: 2)
  541. Efficiency of the transfer in Western Blotting - How can you tell from the gel? (reply: 10)
  542. Western loading control for secreted protein? - (reply: 3)
  543. Western blot internal controls -- Any large proteins? - (reply: 3)
  544. Help with western blotting - protein not running in to seperating gel (reply: 1)
  545. General questions about western - (reply: 2)
  546. Nuclear extract - Protocol for nuclear extract for WB (reply: 2)
  547. western clarification - (reply: 3)
  548. MDR western - (reply: 1)
  549. protein can be found by IHC but not WB - (reply: 2)
  550. Immunoblot (western) resolution vs electrophoresis resolution - (reply: 1)
  551. Tips for Western Blot of Low Molecular Weight Protein - (reply: 4)
  552. Western problem - very challenging - distorted band, non-reproducible, generally awful (reply: 5)
  553. Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use? (reply: 1)
  554. Loading controls for Western blot - (reply: 2)
  555. viewing pentamers on a western blot - (reply: 3)
  556. help on western blots using plasma or serum - (reply: 1)
  557. Same protein, different size on Western and IP ? - (reply: 2)
  558. Western transfer time: Is it crucial for big proteins? - anomaly HELP (reply: 2)
  559. western blot - trouble (reply: 6)
  560. Western blot background and specificity - How good is my antiserum? (reply: 1)
  561. Blank Western blot - (reply: 9)
  562. Western Blot troubleshooting: band is too high - LiCor imaging of Alpha tubulin membranes (reply: 6)
  563. Weak Western band for phosphorylated Akt - whats happening?? (reply: 17)
  564. white bands on western blots - (reply: 1)
  565. western blots and big proteins - (reply: 7)
  566. help - small proteins and western blotting - (reply: 23)
  567. Loss of proteins during Western transfer - (reply: 4)
  568. Cell lysis for westerns? - Immediately after harvest?? (reply: 8)
  569. SDS-PAGE and western blot - (reply: 2)
  570. Where did the protein go? - Low protein reading in WB lysates (reply: 4)
  571. duplet bands in western blot - advice (reply: 5)
  572. Streaks appearing between lanes in my western blot - (reply: 3)
  573. Gel shift assay follow by Western blot analysis - Has anyone try this before? (reply: 8)
  574. Some lanes see bands,other no, strange! - western blotting questions (reply: 10)
  575. Western blotting that doen't work now - original topic (reply: 11)
  576. Western band on blot uncapturable by film - (reply: 3)
  577. How to run SDS-PAGE and Western Blotting for small peptide? - Tricks for polypeptides less than 10KD??? (reply: 5)
  578. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
  579. Western background - (reply: 2)
  580. Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
  581. Western background - vertical stripes ! urgent - (reply: 3)
  582. How to make western results clean??? - (reply: 3)
  583. background in western - very strange stripes ?! - (reply: 2)
  584. siRNA control by Western blot - how do I get equal loading concentration? (reply: 7)
  585. Incubating the filters two overnights with primary antibody. - WESTERN BLOTTING (reply: 3)
  586. Western blotting - (reply: 2)
  587. western blot - problems with detection - (reply: 7)
  588. Western transfer problem - (reply: 3)
  589. Why do I add Tween 20 to my Western Blot? - (reply: 3)
  590. orthovanadate in transfer buffer for WB? - immunodetection of phospho ERK1/2 (reply: 6)
  591. determining protein expression level from a Western!!! - (reply: 6)
  592. Western blot bands look like dumbells - (reply: 2)
  593. western blot trouble shooting - hoeffer products (reply: 1)
  594. Protocol for histone acid extraction for Western blot - (reply: 13)
  595. staining gels and western blot - (reply: 1)
  596. western blotting and histone acetylation - (reply: 2)
  597. Is 100 ug liver tissue protein lysate too much for western blot? - (reply: 2)
  598. Western blot-quantitation - (reply: 4)
  599. problem with stripping membranes of western blott - do you know some efficient stripping buffer (reply: 3)
  600. BSA purity for Western Blot? - What BSA to use? (reply: 1)
  601. Scraping or Trypsinization for westerns?? - (reply: 10)
  602. western blot forgot to transfer - (reply: 2)
  603. WB for Membrane Protein - Not able to see membrane protein on western (reply: 12)
  604. antibody problems in western - Got similiar western signals for three different p (reply: 8)
  605. WB for large proteins - Transfer conditions with PVDF membranes (reply: 5)
  606. WB: one single band but at the wrong size... - what to make of it? (reply: 4)
  607. Western blot of phosphoproteins - (reply: 2)
  608. uneven western blot - (reply: 1)
  609. problem with Protein concentration determination and westernblot - (reply: 11)
  610. <10kD protein in Western - (reply: 3)
  611. Chemiluminescent marker - Western blot problem - (reply: 4)
  612. Western Blot question: cutting membrane into strips - (reply: 2)
  613. Dry membrane to do western blotting - (reply: 4)
  614. Too many bands on Western blot - (reply: 5)
  615. Western Blot: Reducing Buffer Vs Non-erducing buffer - basic Western blot question. (reply: 4)
  616. anti-goat:anti-western blotting??? - Western blotting! (reply: 3)
  617. (Help) Questions about Western - (reply: 6)
  618. protiens with similar MB but shift on the western blot - (reply: 1)
  619. software to quantificate western blot bands - if possible for free!!! (reply: 17)
  620. Distorted bands on Western Blot - (reply: 10)
  621. a question about western blot - question and help western blot (reply: 5)
  622. western troubles - any help with connexins western (reply: 1)
  623. Big problem with Western Blot - Ugly blot (reply: 2)
  624. Western Blots: no signal - low or no detection signal (reply: 8)
  625. possible problem in western blotting/sample preparation?? - (reply: 3)
  626. Protein isolation for western - (reply: 8)
  627. Random Background Noise for Western Blots - I'm getting noise only in some lanes (reply: 1)
  628. western blot samples trouble - (reply: 6)
  629. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
  630. Is it ok to re-use Western Transfer Buffer - (reply: 4)
  631. Help with western blots please - (reply: 5)
  632. Problems in Western blot ! - need help for my western blot (reply: 5)
  633. Tissue prepatation for WB and other issues - (reply: 5)
  634. Sonicating cell lysate for Westerns - (reply: 2)
  635. Western Blot troubleshooting - (reply: 7)
  636. Li-Cor WB optimisation - WB background at y680 of Li-Cor (reply: 1)
  637. whats the problem of my western blot? - (reply: 3)
  638. Problem with TCA and western blot - (reply: 3)
  639. Concentrating protein in cerebrospinal fluid for Western - (reply: 1)
  640. HELP: Western Blot problem - (reply: 3)
  641. Preparation of Cell extracts for Western Blotting? - (reply: 4)
  642. western of a big protein - (reply: 2)
  643. Western Blot stripping - quick and easy protocol? - (reply: 2)
  644. Immunoblot (Western Blot) Question - Trying to nut out a question my demonstrator asked (reply: 1)
  645. Help: western blot lysis buffer - (reply: 2)
  646. Short Half-life of ECL on Western Blot? - Recurrent problem of short ECL half-life (reply: 2)
  647. Western Blot Mystery - Western Blot (reply: 1)
  648. Help on protein integrity Western Blot - Sample preparation and detection (reply: 6)
  649. Western blotting of small proteins - (reply: 3)
  650. can i freeze cell lysates for western blotting? - (reply: 3)
  651. can i freeze supernatants for western blots? - (reply: 3)
  652. question - anti Ha western blotting (reply: 1)
  653. IEF followed by Western blot - problems with poor blotting efficiency (reply: 4)
  654. lysis buffer for western blot - lysis buffer (reply: 3)
  655. Western Blotting - Electrophoretic transfer of proteins (reply: 2)
  656. western blot trouble shoot-plz help - (reply: 5)
  657. troubleshooting westerns - (reply: 1)
  658. Western Blot - (reply: 1)
  659. Western Blot HRP mouse X goat anti human IgG1 - (reply: 3)
  660. enhancing western blot signals - incubation alternatives (reply: 3)
  661. protein quantitation - for equal western loading (reply: 2)
  662. p-Smad2 problems in western blot - TGF-beta and p-Smad 2 (reply: 1)
  663. ABC technique with Western blot - Do you get extra band with ABC? (reply: 1)
  664. Trouble with Western Blot Transfer (Poor transfer) - Could the protein isolation or homogenization be the cause? (reply: 4)
  665. Reuse antibodies for further incubation in Western blot - (reply: 5)
  666. apolipoprotein E identification via Westerns - (reply: 1)
  667. band spread in western - (reply: 9)
  668. Far Western again... - (reply: 1)
  669. Western blot - Stripping and Reprobing - (reply: 18)
  670. Far western - (reply: 3)
  671. p16 antibody in western blotting, weird results - western blotting, p16 (reply: 4)
  672. Western blot detection other than antibody? - (reply: 5)
  673. Problems with phospho-MAP and phospho-FAK - No signal by WB. (reply: 1)
  674. Western blot problem - Western blot (reply: 5)
  675. Western Blot "Strange Results" Please help - (reply: 4)
  676. loading control for western blots - (reply: 1)
  677. Western Blot Loading Control - (reply: 2)
  678. Putting more than one membrane in a container... - Western (reply: 2)
  679. Western Blot - Semidry Transfer (reply: 3)
  680. How to determine band intensity of Western blot? - High School Student in need of help for the Science Fair! (reply: 8)
  681. Strange problem with actin control in Western blot - actin (reply: 3)
  682. Bands on Western are not sharply defined - (reply: 6)
  683. Is adding reducing agent necessary before protein boil? - For Western blot (reply: 4)
  684. phastgels for Western blotting - (reply: 1)
  685. Western blot stripping problem - (reply: 6)
  686. Western for membrane bound receptor - (reply: 1)
  687. Western Blot Densitometry - (reply: 2)
  688. Western blot - Pounceau S staining problem - (reply: 16)
  689. Western problem - 8 months now...(long) - (reply: 2)
  690. western blot - transfer trouble (reply: 5)
  691. Western blot mystery - (reply: 2)
  692. total protein in Western blotting - (reply: 1)
  693. Western Primary AB - (reply: 2)
  694. semiquantitative western blotting - (reply: 1)
  695. Whole Blood storage - How to store whole blood for Western analysis (reply: 1)
  696. bone for western blotting - bone processing for western blotting (reply: 4)
  697. Protein solubilization and Western Blotting - (reply: 1)
  698. ECL Western blotting detection kit - (reply: 5)
  699. Membrane Protein Western Blot. - Troubleshooting western blot (reply: 4)
  700. densitometry of westerns - (reply: 4)
  701. Odd Western Blotting problems - (reply: 4)
  702. western blotting with anti-goat HRP - bands after ECL but only secondary antibody (reply: 1)
  703. Western blot problem: not running in gel - western blot troble (reply: 7)
  704. Nonspecific Binding on Western Blot - HELP (reply: 10)
  705. western blotting question - (reply: 3)
  706. Extraction of membrane proteins and western on them - (reply: 3)
  707. Western Blotting Spotting - Spots on Westerns Blots (reply: 5)
  708. MAPK P38 - troubles with western blot (reply: 8)
  709. Another Problem with Western blot. - (reply: 3)
  710. Western Blot - signal doesn't show up on film - (reply: 8)
  711. Western Blots - (reply: 1)
  712. Expression of Proteins for western blot - (reply: 1)
  713. Western - X-reaction with molecular weight marker (all of a - (reply: 1)
  714. Western imaging analysis - (reply: 1)
  715. Major problem with Western blotting - (reply: 2)
  716. Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
  717. Western blot - Reversed polarity (reply: 3)
  718. too many bands in western blotting - (reply: 2)
  719. Western Blot Transfer Problem - no more markers! - did they 'blow through?"Help! (reply: 2)
  720. western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
  721. Western Blotting Help!!!! - Problem with an internal control (reply: 7)
  722. western blotting using alkaline phosphatase - (reply: 4)
  723. Western Blotting Problem - High band (reply: 1)
  724. western blotting - (reply: 1)
  725. western blotting of expressed protein - (reply: 1)
  726. IP/Western complication - Trying to pull down 2 conjoined proteins (reply: 2)
  727. Western and sample boiling - (reply: 1)
  728. Formalin Fixed Tissue westerns - (reply: 1)
  729. Western blotting background troubleshooting - (reply: 64)
  730. Western blot question - (reply: 1)
  731. detection threshold of western blotting - (reply: 3)
  732. High background in Western blot - (reply: 28)
  733. western blot for membrane protein with his-tag - can't detect with anti-his tag Ab? (reply: 3)
  734. western blot problem - Blank film from WB with ECL (reply: 2)
  735. semi-dry western conditions - (reply: 20)
  736. Double Bands in Western Blotting - Please Help (reply: 2)
  737. cell Transfection and western blot - transfection and western (reply: 2)
  738. Western blot for 2D gel - (reply: 1)
  739. Western blotting quantification and normalisation - (reply: 2)
  740. Western blotting quantification and normalisation - (reply: 3)
  741. Wester VS Immunoprecipitation -Da Paradigm...?!?! - Western Blot & Immunoprecipitaion & Ab (reply: 3)
  742. TCR Zeta Chain & Phospho-species - immunoprecipitation and WB (reply: 1)
  743. western blotting - dab (reply: 3)
  744. aspecific band of BSA in western blot - western blotting (reply: 1)
  745. blocking agent for phosphoproteins - options for westerns used to detect phos (reply: 1)
  746. Has anybody done western blot on calmodulin? - (reply: 1)
  747. Help! Western Blot - (reply: 2)
  748. Western Blot - Cross reactivity of first Ab (reply: 1)
  749. Western blot doesn't work anymore - low sensitivty in Western blots (reply: 1)
  750. Neg control fopr western? - (reply: 3)
  751. Western Blot - How to improve transfer on the sides? (reply: 1)
  752. Western Blot for Elastin orother Matrix Proteins - (reply: 1)
  753. His-tag purification and his-antibody for western - (reply: 2)
  754. Western blotting - protein quantification and free-software (reply: 2)
  755. western blot - (reply: 4)
  756. How the West(ern) was lost - (reply: 3)
  757. MHC Class I Western blotantibody??? - (reply: 1)
  758. VEGF Western blots - (reply: 4)
  759. Transfection and Western - (reply: 2)
  760. Western Blotting - (reply: 4)
  761. problem with trizol - solubilization and western of proteins (reply: 2)
  762. labeling on southern, northern and western blot - (reply: 1)
  763. How to concentrate protein from culture medium - for Western blot (reply: 1)
  764. about Western Blot - (reply: 3)
  765. eliminating albumin from samples for western blotting - (reply: 1)
  766. Trouble detcting phosphJNK by western - (reply: 1)
  767. question for western blotprofessionals - (reply: 2)
  768. Electroblotting of proteins(western) - (reply: 2)
  769. Western Blotting. - How to reduce non-specfiic bound signals?? (reply: 8)
  770. Western - (reply: 2)
  771. His-tag Westerns: Bestcommercial antibody - (reply: 1)