WB of beta amyloid - a simple method for WB to visualize b-amyloid? (Nov/10/2008 )

Hi,
I am new in beta-amyloid field.. I am interested in running a SDS gel to visualize b-amyloid. I have found several methods, but I cannot make many trials as I dont have many samples. Do you think a 16.5% of Tris-Glycine, 120 V, is enough?
Do I have to take care about boiling, SDS, beta-mercaptoethanol...I bought a color marker to avoid running off my beta-amyloid.
Thanks a lot!

-inmaculada-

you should use tris-tricine.

you do need to use sds, 2-me. a-beta is "sticky", you need to ensure that the peptide remains a monomer.

-mdfenko-

Thanks
I attach a very good paper about Tricine gels.
I have a Bio-Rad Protean II Unit for electrophoresis (the most common, I guess) Once I have mounted the gels..do I have to put the cathode buffer inside the basket with the gels? (so the anode will be outside)

thanks again

-inmaculada-

the cathode buffer goes into the buffer well that covers the gel's teeth (ie where you load the sample).

the proteins will travel from the cathode to the anode.

-mdfenko-