Western transfer problem - (Aug/31/2005 )

I have a favor and question to ask .....
NC paper and PVDF which one have a better tranfer efficiency ?
I usually use 100V(about 300-400mA) for 4hours tranfer ,sometimes 50V for overnight transfer.
but lots of proteins are always left on the SDS-PAGE.
what can i do to reslove this probelm?
Any suggestion will be highly appreciated.
thank you by wenni

What is the recipe of your transfer buffer ?

try longer transfer times, or if possible use a lower percentage acrylamide gel. Do all the proteins transfer with the same effeciency, or do the lower mw proteins transfer better? I often don't get good transfers of proteins above 150 kDa with 15% acrylamide gels . ( I'm using these gels to study low mw proteins 6-20 kDa , but my standards from Biorad include some high weight prestained proteins that I see trapped in the gel after transfer)
Also, if you used correctly, I don't think you would see much difference between results from PVDF or nitrocellulose.
monkeybaji