What high molecular weight loading control for Western blotting? - (Jul/03/2008 )

Hi All,
Could anyone suggest a higher molecular weight loading control, ( 75-200 kDa), and my samples are rat cardiomyocyte membranes. Your help is appreciated.
THanks
Tulip

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Invitrogen has a special marker for "large" proteins, the highest fragment is 460 kDA (depending on the gel offcourse) and the lowest 31 kDa. I find it a nice one.
invitrogen himark marker

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Thanks for the reply, but I meant loading control as beta actin or tubulin and not the protein standard.

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Ow, I'm sorry. When I'm blotting for collagen (140 kDa, 280kDa and 420 kDa fragments), I use beta-actin as a loading control. On a 3-8% tris-acetate gel, the collagen runs until half of the gel and the beta actin is than on about one quarter away from the end of the gel. I don't know if there is a better loading control that is higher?

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