what is the equation for quantitative western blot - (Apr/29/2008 )

I'm comparing the expression of a protein in my treated animals and controls by western blot. So the way I do it is that I scan the bands ( target protein and actin), measure the color intensity in each band, then put it in this equation; target/actin in treated animals divided by target/actin in control animals. I think in this way I can have a good understanding of the net increase in my target protein. Does it make sense? Do you know any documented equation for that purpose?

What you have described is correct. I am unsure if this is documented ... Google it.

Fundamentals of densitometry:
http://rsb.info.nih.gov/nih-image/more-doc...ng/ImgEngr.html

One of the methods to quantitate the western result: http://www.lukemiller.org/journal/2007/08/...ts-without.html