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lysate for ELISA can be used for WESTERN? - (Jun/13/2006 )

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Hi, I am a new one for ELISA.

Currently I am considering making protein from liver for ELISA and Western-blot. As the amount of my sample is very limited, if I prepare the lysate for ELISA, can I also use those lysate for Western?

Thanks for your information!

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-BLUE-SNOW-

I guess it should work.

-scolix-

hi BLUE-SNOW ! that depends on the type of detergent you are using for the lysis. but most protocols suggest a non ionic detergents and this works well for both ELISA as well as western blotting
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-SHIVA KESHAVA-