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Vector Related Discussions
  1. Transformation and plasmid extraction - (reply: 1)
  2. Erratic Cq values from Plasmids - (reply: 5)
  3. Storing plasmid at 4 degrees C ?. - (reply: 2)
  4. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
  5. How to make an empty vector (such as pET ecoli vector) using plasmid miniprep an - (reply: 1)
  6. plasmid purification - (reply: 4)
  7. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
  8. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
  9. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
  10. low copy number plasmid purification kit - (reply: 4)
  11. String of A's (15-20nt) w/out polyA signal in middle of rlentiviral vector; - (reply: 1)
  12. Help Needed Finding Some Plasmid Prep Protocols - (reply: 2)
  13. Crude lysis Plasmid isolation - (reply: 1)
  14. Plasmid analysis in limited conditions - (reply: 6)
  15. Plasmid distributors for Luciferase Assay? - (reply: 2)
  16. pGL3+promoter vector problem - (reply: 1)
  17. Ligate a very small insert into a vector - (reply: 3)
  18. Viscous plasmids and transfection issues - (reply: 2)
  19. Plasmid dna size - (reply: 3)
  20. When overexpressing several genes using different vectors, what negative control - (reply: 2)
  21. pYr-CMV-Kan2 vector - (reply: 1)
  22. What is your favorite method for getting inserts into vectors? - (reply: 3)
  23. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
  24. Pipeline to search bacterial CRISPR spacers against specific phage genomes? - (reply: 1)
  25. GFP tagged plasmid - (reply: 1)
  26. Designing primers with his-tag for pET28a+ vector - (reply: 3)
  27. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  28. Plasmid purification for Gibson Assembly - (reply: 2)
  29. Co-transfection of multiple plasmids - (reply: 1)
  30. pKD1 based vector for K. lactis expression - (reply: 2)
  31. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  32. what‘s phage display principle - (reply: 1)
  33. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  34. pET32b+ vector issue - (reply: 1)
  35. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  36. How stable is double digested plasmid - (reply: 7)
  37. Mature miRNA in expression vector? - (reply: 1)
  38. Ligation: insert & vector are about the same size - (reply: 3)
  39. low plasmid concentration - (reply: 1)
  40. What make a plasmid candidate for stable transfection - (reply: 1)
  41. Lower Plasmid Yields - (reply: 5)
  42. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  43. Question on plasmids - (reply: 4)
  44. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  45. pUC and pSC101 vectors in the same cell - (reply: 3)
  46. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  47. Antisense vectors confused about construction - (reply: 1)
  48. Double Digest pCD Vector - (reply: 3)
  49. Double digestion problem with PUCsp vector - (reply: 1)
  50. How much plasmid for transformation? - (reply: 8)
  51. replicating a mammalian expression vector in bacteria - (reply: 3)
  52. preparing a vector for transfection - (reply: 2)
  53. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  54. Suitable ratio of vector and insert in cloning - (reply: 5)
  55. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  56. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  57. plasmid repositories - (reply: 2)
  58. How to insert PreScission protease into a vector - (reply: 1)
  59. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  60. real time qPCR standard range plasmid - (reply: 3)
  61. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  62. Cloning a big insert in a small vector - (reply: 2)
  63. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  64. Plasmids recombining - (reply: 3)
  65. Plasmid Methylation - (reply: 3)
  66. Why are there two origin of replications in plasmids - (reply: 3)
  67. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  68. Plasmid Amplification Issue - (reply: 6)
  69. ratios insert/vector - (reply: 3)
  70. Swapping promoter of destination vector not working. Please help! - (reply: 2)
  71. IRES in expression vector - (reply: 1)
  72. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  73. Can't isolate plasmid from cloned E.coli - (reply: 4)
  74. Denatured plasmid for pcr - (reply: 1)
  75. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  76. Problems with M13 phage - (reply: 5)
  77. Screen for F plasmid in e. coli - (reply: 1)
  78. Plasmid Linearization Problems - (reply: 9)
  79. Changing the vector - (reply: 55)
  80. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  81. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  82. plasmid construction - (reply: 2)
  83. vector propagation in E coli - (reply: 1)
  84. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  85. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  86. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  87. direction of the expression units in multi-gene vectors - (reply: 1)
  88. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  89. ethanol precipitation plasmid DNA - (reply: 3)
  90. Plasmid storage conditions - (reply: 5)
  91. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  92. Temperature sensitivity of Plasmids - (reply: 1)
  93. New (free) plasmid mapping program - (reply: 4)
  94. filling the digested vector and insert for blunt end cloning - (reply: 2)
  95. removing part from plasmid - (reply: 18)
  96. Help with plasmid extraction and redissolving? - (reply: 5)
  97. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  98. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  99. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  100. Promoter sequence search in a plasmid map - (reply: 3)
  101. Heat vs pH during plasmid extraction - (reply: 8)
  102. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  103. Heating elution buffer for plasmid prep - (reply: 1)
  104. Cloning into pVIVO2 vector - (reply: 8)
  105. Cloning worked, but the expression vector is weird - (reply: 6)
  106. Using pBABE plasmids for regular transfection - (reply: 1)
  107. Rxn not working - amplify/linearize plasmid - (reply: 3)
  108. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  109. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  110. RNA contamination in plasmid extracted - (reply: 1)
  111. Problem in plasmid digestion after transformation - (reply: 4)
  112. Call for help! Plasmid extraction problem - (reply: 4)
  113. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  114. How can addgene sell plasmids with patented technology - (reply: 2)
  115. Transient transfection with large plasmid - (reply: 3)
  116. How to obtain Vectors used in articles - (reply: 5)
  117. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  118. Gateway vectors - (reply: 3)
  119. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  120. Plasmids DNA on filter paper - (reply: 1)
  121. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  122. Kozak sequence and mammalian expression vector - (reply: 3)
  123. GFP vector - (reply: 1)
  124. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
  125. Problem with Large vector & very small insert - (reply: 1)
  126. Storage life of plasmid DNA in water - (reply: 5)
  127. Insert & vector too dilute to perform ligation - (reply: 3)
  128. Plasmid DNA digestion problem - (reply: 3)
  129. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  130. free online DNA & plasmid editing tool - (reply: 1)
  131. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  132. Appearance of a salty plasmid preparation - (reply: 1)
  133. Common RE site on complementary strands in a Vector - (reply: 7)
  134. Transformation of Restricted plasmid - (reply: 3)
  135. Why is my insert missing from my plasmid? - (reply: 4)
  136. Plasmid digestion and gel purification using HindIII - (reply: 3)
  137. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  138. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  139. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  140. Colonies grown without plasmid - (reply: 7)
  141. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  142. Plasmid on blotting paper - (reply: 1)
  143. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  144. vector dephosphorylation - (reply: 3)
  145. What plasmid sizes do you work with for SDM? - (reply: 1)
  146. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  147. Adshuttle vectors and adenoviruses - (reply: 1)
  148. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  149. cloning help; high salt level in vector - (reply: 4)
  150. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  151. How to get rid of bacterial phage from a caterpillar surface? - (reply: 7)
  152. Running undigested plasmids on gel - (reply: 2)
  153. Plasmid isolation from Enterococcus - (reply: 1)
  154. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  155. Software for vector sequence manipulation - (reply: 2)
  156. overexpress mature microRNA by siRNA vector - (reply: 2)
  157. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  158. Whole plasmid amplification by PCR - (reply: 2)
  159. For promoter assay, which vector I should use? - (reply: 5)
  160. Sequencing of the cloned plasmid - (reply: 5)
  161. 4 way cloning into TA Vector - (reply: 10)
  162. Plasmid DNA contamination - (reply: 5)
  163. Problems with plasmid DNA extraction - (reply: 3)
  164. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  165. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  166. Can I quantify restricted plasmid and oligos? - (reply: 3)
  167. conditionally active plasmids - (reply: 1)
  168. Transient transfection of linearized plasmids - (reply: 3)
  169. Transfecting three plasmids into hES cells - (reply: 2)
  170. HUVEC plasmid transfection challenges - (reply: 2)
  171. Plasmid DNA - (reply: 3)
  172. Electroporate lentiviral vector for stable cell line - (reply: 2)
  173. Genomic DNA and plasmid DNA isolation - (reply: 3)
  174. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  175. faint band after plasmid extraction - (reply: 7)
  176. pTRIPZ lentiviral vector induction - (reply: 1)
  177. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
  178. plasmid extraction troubleshooting,please help !! - (reply: 9)
  179. plasmid digestion - problem with enzymes? - (reply: 4)
  180. Plasmid stability in Ecoli during storage - (reply: 3)
  181. which to use: miR or miRexpression vector - (reply: 1)
  182. Ligation with small insert and large vector - (reply: 6)
  183. Lost of Tet-on Vector - (reply: 3)
  184. Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
  185. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  186. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
  187. Determining in frame in modified vector - (reply: 1)
  188. Creating primers to add restriction sites to vector backbone - (reply: 7)
  189. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  190. Low copy vector for library generation - (reply: 2)
  191. Plasmid isolation from gram positive bacteria - (reply: 2)
  192. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  193. problems with plasmid miniprep - (reply: 8)
  194. shRNA interference using Lenti virus vector - (reply: 1)
  195. Determining the amount of plasmid transfected - (reply: 1)
  196. How to check for the presence of plasmid? - (reply: 3)
  197. PCR with Plasmid recovered from filter paper - (reply: 6)
  198. Check contamination - how to sterilize vector - (reply: 1)
  199. Help for pGEM-T easy vector ligation problem - (reply: 2)
  200. pkp 59 vector - (reply: 2)
  201. Vector insert ratio in cloning - (reply: 3)
  202. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  203. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  204. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  205. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  206. restricted PCR plasmid runs slower - (reply: 2)
  207. High frequency of vector religation - (reply: 10)
  208. Plasmid compatibly groups - (reply: 5)
  209. Plasmid isolation from agrobacterium - (reply: 1)
  210. Cutting my plasmid made it larger - (reply: 7)
  211. What are the "mammalian vectors N1 and C1"? - (reply: 1)
  212. Packaging vectors - (reply: 1)
  213. Recreate original plasmid by cutting out insert - (reply: 2)
  214. posting sequences of plasmid? - (reply: 3)
  215. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  216. problem with plasmid digestion - (reply: 5)
  217. Protein Expression vector with small tag - (reply: 1)
  218. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  219. unexpected band in PCR with plasmid - (reply: 4)
  220. Sequencing Primers and Plasmids - (reply: 3)
  221. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  222. Replication of the Plasmid DNA - (reply: 6)
  223. expression plasmids for tRNA - (reply: 6)
  224. cloning pcr insert into plasmid - (reply: 2)
  225. Re-culturing empty plasmid - (reply: 1)
  226. microRNA promoter based expression vector? - (reply: 1)
  227. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  228. help with a vector - (reply: 3)
  229. Adding T7 promoter sequence to vector - (reply: 2)
  230. Plasmid Miniprep - (reply: 2)
  231. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  232. Tricistronic vector - (reply: 1)
  233. phage lysate purification - (reply: 2)
  234. Plasmid DNA at RT for a few days - (reply: 4)
  235. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  236. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  237. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  238. Lentviral vectors - (reply: 1)
  239. plasmid rearrangement or sabotage - (reply: 2)
  240. pLDR20 Vector - (reply: 1)
  241. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  242. Linearising plasmid before transfection? - (reply: 2)
  243. Smear in plasmid sample - (reply: 9)
  244. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  245. vector dephosphorylation - (reply: 5)
  246. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  247. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  248. cloning a gene in to a plasmid - (reply: 5)
  249. Characterizing an unknown plasmid - (reply: 6)
  250. Plasmid Purification and Digestion Problem - (reply: 2)
  251. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  252. QuikChange with Different plasmids - (reply: 1)
  253. Co-transfection with 2 plasmids - (reply: 10)
  254. Vector Digestion Problem - (reply: 4)
  255. Single Plasmid Gel Band - (reply: 1)
  256. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  257. Transformation with a big plasmid - (reply: 4)
  258. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  259. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  260. digested plasmid - (reply: 2)
  261. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  262. isolation of plasmid (midi and maxi prep) - (reply: 18)
  263. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  264. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  265. pUB Vector with two promoters - (reply: 6)
  266. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  267. pkD46 vector growth in E.coli problem - (reply: 8)
  268. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  269. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  270. plasmid tetR and tet promoter - (reply: 2)
  271. Trouble in phage display - (reply: 1)
  272. Plasmid clone in BSP - (reply: 1)
  273. Swich selection in plasmid - (reply: 3)
  274. A good vector to make gene library? - (reply: 5)
  275. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
  276. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  277. Hypothetical Synthetic Plasmid - (reply: 9)
  278. How many fragments in plasmid DNA prep? - (reply: 1)
  279. Problem with plasmids transformation - (reply: 6)
  280. Calcium chloride plasmid transfection - (reply: 1)
  281. How Much Plasmid? - (reply: 6)
  282. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  283. Plasmid isolation - (reply: 1)
  284. plasmid；GI; ACESSION NUMBER - (reply: 2)
  285. virus transduction vs plasmid transfection - (reply: 4)
  286. How dangerous are non-viral vectors? - (reply: 3)
  287. having trouble getting insert ligation into vector - (reply: 3)
  288. Bizarre changes in plasmid band size - (reply: 3)
  289. where to get vector overexpressing caveolin-1? - (reply: 2)
  290. plasmid repository - (reply: 2)
  291. LZRS vector details - (reply: 1)
  292. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  293. 12.4kb vector 6.7kb insert - (reply: 2)
  294. pGEM-T Easy vector contamination - (reply: 2)
  295. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  296. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  297. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  298. I need bynary vector pART27 - (reply: 1)
  299. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  300. pCCL vector map - (reply: 2)
  301. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  302. Pls help me with this plasmid!!! - (reply: 1)
  303. Standard curve using plasmid - (reply: 1)
  304. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  305. Methods of plasmid transformation? only heat shock? - (reply: 1)
  306. Cloning into inducible vector but no expression - (reply: 1)
  307. e coli vector and host selection - (reply: 1)
  308. No plasmid after a maxi prep mystery - (reply: 4)
  309. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  310. Co-expression of 2 plasmids in E.coli - (reply: 1)
  311. How to avoid plasmid instability - (reply: 4)
  312. Plasmid vs cDNA amplification by PCR - (reply: 2)
  313. Shear-induced damage of plasmid by vortexing - (reply: 1)
  314. RNase Band in old plasmid stored at -40 - (reply: 2)
  315. Plasmid vector for yeast and E. coli - (reply: 2)
  316. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  317. pGEX vector - (reply: 2)
  318. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  319. Filamentous phage infection - (reply: 2)
  320. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  321. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  322. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  323. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  324. Plasmid digestion after transformation - (reply: 4)
  325. Blunt end ligation insert:vector ratio - (reply: 1)
  326. Smear above plasmid dna - (reply: 3)
  327. result of plasmid extraction - (reply: 2)
  328. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  329. plasmid extraction - (reply: 5)
  330. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  331. plasmid extraction - (reply: 3)
  332. Buy BAC vectors - (reply: 1)
  333. Is that a Plasmid??? - (reply: 5)
  334. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  335. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  336. plasmid extraction - (reply: 13)
  337. ligation colonies contain empty vector.. - (reply: 9)
  338. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  339. Cloning two genes in the same plasmid - (reply: 4)
  340. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  341. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  342. Plasmid, gene orientation, insert orientation. - (reply: 1)
  343. Plasmid recovery problem - (reply: 3)
  344. Cloning problem using pET32a vector - (reply: 3)
  345. Electroporated plasmid loses the insert - (reply: 8)
  346. emty vector cloning - (reply: 3)
  347. Plasmid sequencing without primers - (reply: 1)
  348. RE site on complimentary strand of plasmid? - (reply: 1)
  349. How much of plasmid should be cut to see the gene - (reply: 1)
  350. Plasmid sequencing - (reply: 1)
  351. Plasmid isolation Qiagen - (reply: 2)
  352. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  353. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  354. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  355. Help in RE digestion of vector - (reply: 5)
  356. Amplify fragment in plasmid? - (reply: 4)
  357. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  358. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  359. Gateway cloning vector compatibility - (reply: 2)
  360. gene expression in E coli with PET21a vector - (reply: 1)
  361. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  362. Template DNA (plasmid) concentration for PCR - (reply: 4)
  363. co-expression of 2 plasmids in E.coli - (reply: 3)
  364. How to choose the right plasmids for certain types of cell? - (reply: 1)
  365. Plasmid digestion create a bigger plasmid - (reply: 1)
  366. Cloning two fragments into a vector - (reply: 3)
  367. confirmation of insert in to vector - (reply: 3)
  368. whole plasmid pcr? - (reply: 6)
  369. Looking for a GFP plasmid (non-amp) - (reply: 3)
  370. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  371. ligation of pcr product into expression vector - (reply: 4)
  372. amp plates containing xgal/iptg with expression vector - (reply: 6)
  373. Strange Vector Problems - (reply: 3)
  374. Insert lose in Phage display library - (reply: 6)
  375. extract plasmids from transfected eukaryotic cells - (reply: 6)
  376. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  377. Lenti vector ligation - (reply: 2)
  378. Cloning from a pGEMT Easy vector - (reply: 3)
  379. Plasmid compatible with pBAD24 - (reply: 1)
  380. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  381. COL E1 mutations in vector after sequencing - (reply: 1)
  382. Vector with inducible/repressible system - (reply: 1)
  383. Improving expression of recomb proteins in baculovirus - (reply: 1)
  384. Cloning genes into plant vector - (reply: 1)
  385. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  386. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  387. Recombinant construct looks smaller than empty vector - (reply: 3)
  388. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  389. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  390. protein expression vector.. - (reply: 4)
  391. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  392. Lentiviral Vector - Lentiviral Vector (reply: 5)
  393. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  394. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  395. Best transcription vector - (reply: 1)
  396. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  397. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  398. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  399. Cloning into lentiviral vector - (reply: 11)
  400. use of pGEM -tT easy vectors - (reply: 2)
  401. ~200kb Plasmid DNA purification is not working - (reply: 1)
  402. ligating a 6 His tag to plasmid - (reply: 2)
  403. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  404. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  405. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  406. Plasmid sequencing failed why? - (reply: 10)
  407. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  408. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  409. SLIM mutagenesis of large plasmids - (reply: 8)
  410. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  411. Can you receommend a vector? - (reply: 1)
  412. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  413. why 12 h culture for plasmid prep? - (reply: 2)
  414. Big plasmids and Transfection - (reply: 3)
  415. How to add a T7 terminator to a vector? - (reply: 4)
  416. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  417. Protein expression vectors Please help - (reply: 2)
  418. vectors please help - vectors cleavage sites (reply: 1)
  419. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  420. very small insert and a very ling vector - (reply: 1)
  421. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  422. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  423. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  424. pcr amplify an insert in plasmid? - (reply: 2)
  425. Single-digested vector shifts downward on gel - (reply: 1)
  426. odd plasmids - (reply: 2)
  427. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  428. Lactobacillus plasmid - (reply: 1)
  429. Will transfected plasmids induce cytokine production? - (reply: 1)
  430. Question about plasmid midiprep - (reply: 1)
  431. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  432. Mirror Image Phage Display - "special" contamination (reply: 9)
  433. pGL3 vector - (reply: 3)
  434. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  435. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  436. Overexpression plasmid is not expressing protein - (reply: 13)
  437. Secretion cloning vectors - (reply: 1)
  438. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  439. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  440. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  441. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  442. PCR problem - basic PCR for plasmid amplification (reply: 2)
  443. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  444. New to Gene cloning - Plasmid vector preparation (reply: 2)
  445. cloning a 34kb insert - please suggest good vectors (reply: 1)
  446. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  447. How long can I store cleaved/digested vector? - (reply: 9)
  448. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  449. Low yields of plasmids from miniprep - (reply: 1)
  450. A gene is not inserted into the Plasmid - (reply: 2)
  451. purification of PCR product for cloning in a vector - (reply: 3)
  452. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  453. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  454. Plasmid Question - (reply: 4)
  455. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  456. Weird migration of double digested plasmid - (reply: 4)
  457. pMX-PURO Vector not growing - (reply: 3)
  458. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  459. Help for co-transfection of proteins and plasmid - (reply: 1)
  460. subcloning into bacterial expression vector - (reply: 1)
  461. Calculating MOI of viral vector - (reply: 1)
  462. cloning into F1 ori of expression vector??? - (reply: 1)
  463. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  464. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  465. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  466. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  467. Separating cut/non-cut vector - (reply: 6)
  468. ligating small inert into a large vector - (reply: 4)
  469. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  470. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  471. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  472. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  473. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  474. Undigestable/unrestrictable Plasmid? - (reply: 1)
  475. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  476. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  477. metagenomic library consturction - BAC vectors (reply: 1)
  478. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  479. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  480. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  481. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  482. pGEX vectors and GST expression - (reply: 3)
  483. problems encountered when working on a large plasmid - (reply: 2)
  484. expression vector with built in promoter - (reply: 1)
  485. make a Mega plasmid mutation - (reply: 1)
  486. Anyone make their own PCR cloning vector? - (reply: 5)
  487. Bicistronic vector vs "normal" vector - (reply: 5)
  488. Can't seem to get insert into vector... please help - (reply: 4)
  489. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  490. Plasmid standard problem - (reply: 2)
  491. Do plasmids work in bacteria? - (reply: 1)
  492. CEN sequences on plasmids (yeast) - (reply: 4)
  493. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  494. Plasmid out of BL21 for mutagensis - (reply: 3)
  495. ORI names - name of a plasmid ori (reply: 11)
  496. uncut vector contamination in gel purified band - (reply: 2)
  497. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  498. Change origin of a plasmid - (reply: 4)
  499. Better to sequence from plasmid? - (reply: 5)
  500. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  501. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  502. pGL3 promoter vector - (reply: 1)
  503. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  504. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  505. DNA extraction from induced phage - (reply: 1)
  506. How to get rid of phage contamination - (reply: 1)
  507. lambda phage library - (reply: 4)
  508. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  509. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  510. problem with pLK01 plasmid ligation - (reply: 2)
  511. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  512. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  513. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  514. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  515. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  516. Dephosphorylation of vector - (reply: 8)
  517. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  518. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  519. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  520. PCR mutagenesis of plasmid - (reply: 5)
  521. Guanadine HCl for plasmid miniprep - (reply: 3)
  522. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  523. Large vector cloning-recombination problem? - (reply: 1)
  524. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  525. Not getting plasmid from miniprep - (reply: 7)
  526. Need advice on cloning very small insert into a vector - (reply: 3)
  527. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  528. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  529. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  530. Digesting large amount of plasmid - (reply: 4)
  531. Vectors with special MCS - (reply: 5)
  532. Cloning <100 bp fragments into a vector - (reply: 7)
  533. Cloning <100 bp fragments into a plasmid - (reply: 7)
  534. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  535. Help! my plasmid size changed after ligation - (reply: 5)
  536. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  537. Restriction digestion of gDNA for phage library - (reply: 5)
  538. mammalian expression vector - (reply: 2)
  539. Electroporation doesn't like my plasmid??? - (reply: 4)
  540. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  541. Cloning in pCR2.1 plasmid - (reply: 7)
  542. No expresion of transgene - Retroviral vector (reply: 1)
  543. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  544. lambda phage library - (reply: 1)
  545. More colonies fron vector only control? - (reply: 1)
  546. empty vectors more than the insert - (reply: 6)
  547. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  548. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  549. phage display - peptide differentiation (reply: 1)
  550. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  551. ligation of an adaptor into a vector - (reply: 4)
  552. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  553. vectors and his-tags - (reply: 3)
  554. miRNA 3´UTR target expression vectors - (reply: 6)
  555. Help cloning large insert into large vector - (reply: 7)
  556. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  557. problem about vector Dephosphorylation - (reply: 2)
  558. Cloning: Randomly recombined Plasmids? - (reply: 6)
  559. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  560. plasmid + sepharose - how do this work? (reply: 1)
  561. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  562. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  563. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  564. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  565. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  566. Plasmid size problems! - (reply: 3)
  567. Left DNA plasmid out for a week - (reply: 3)
  568. Vector maps - (reply: 3)
  569. Transfecting primary cells with plasmid - (reply: 1)
  570. question about plasmid extraction - (reply: 2)
  571. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  572. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  573. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  574. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  575. Disappearing plasmid - (reply: 5)
  576. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  577. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  578. problem in plasmid transfection - (reply: 2)
  579. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  580. Plasmid DNA mini-prep - no good isolation (reply: 5)
  581. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  582. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  583. plasmid extraction - why dilute (reply: 3)
  584. Suicide vector - (reply: 2)
  585. very high molecular weight plasmid - (reply: 6)
  586. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  587. vectors maps - (reply: 2)
  588. How to dissolve phage library dry powder - (reply: 2)
  589. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  590. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  591. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  592. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  593. Concepts behind plasmid prep - (reply: 2)
  594. help with pCMV-myc vector - (reply: 2)
  595. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  596. PCR off plasmid for screening - (reply: 2)
  597. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  598. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  599. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  600. Vector frameshift problem - (reply: 3)
  601. Plasmid frameshift problem after clonning - (reply: 2)
  602. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  603. plasmid extraction kit wash buffer solution - (reply: 3)
  604. plasmid extraction kit solution composition - wash buffer (reply: 2)
  605. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  606. are plasmids used for lab work methylated - (reply: 1)
  607. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  608. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  609. Isolation of protein crosslinked to plasmid - (reply: 3)
  610. Inseparable insert in gel purification of the vector - (reply: 3)
  611. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  612. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  613. Which Plasmid DNA for standard curve? - (reply: 5)
  614. pRL-TK as primary Vector ? - (reply: 1)
  615. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  616. GFP insert - trying to insert GFP in a vector (reply: 1)
  617. Plasmid problem - From PCR product (reply: 7)
  618. IFU, MOI, lentiviral vectors - (reply: 1)
  619. viral vector - retroviral vectors (reply: 1)
  620. Gateway and Entry Vectors. - (reply: 1)
  621. Checking PCR insert (into pGEMT vector) - (reply: 2)
  622. Transfection - Plasmid Uptake Control - (reply: 2)
  623. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  624. Culture Grow well but No Plasmid - (reply: 2)
  625. software for plasmid drawing - (reply: 13)
  626. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  627. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  628. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  629. plasmid purification kit for transfection - (reply: 2)
  630. best plasmid purification kit for transfection - (reply: 1)
  631. Expression problem using invitrogen pRSET C vector - (reply: 5)
  632. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  633. Which pGL4 vector to choose - (reply: 2)
  634. Vector containing N-his tag and TEV - (reply: 1)
  635. LR reaction - Cloning into a expression vector (reply: 1)
  636. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  637. Checking Plasmid Expression - (reply: 1)
  638. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  639. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  640. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  641. PCR a plasmid protein - (reply: 2)
  642. plasmid DNA disappear in the minipre - (reply: 2)
  643. Interpreting a Plasmid Profile gel photo - (reply: 3)
  644. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  645. marker swap plasmid - (reply: 4)
  646. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  647. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  648. plasmid dna isolation - (reply: 1)
  649. Vector needed - (reply: 1)
  650. having the wrong vector in my clone - (reply: 3)
  651. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  652. siRNA and plasmid cotransfection - (reply: 1)
  653. rna from plasmid - (reply: 1)
  654. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  655. Problems facing in cloning with pBI121 vector - (reply: 1)
  656. design parameters for multiple siRNA expression in vector - (reply: 4)
  657. Plasmid curing method - (reply: 4)
  658. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  659. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  660. religate the vector - need good protocol! - (reply: 4)
  661. plasmid DNA lab question - (reply: 11)
  662. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  663. Problem amplifying plasmid - (reply: 1)
  664. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  665. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  666. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  667. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  668. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  669. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  670. What about the Baculovirus expression system? - (reply: 2)
  671. Vector NTI from Invitrogen - (reply: 4)
  672. role of glucose in plasmid isolation? - (reply: 1)
  673. Plasmids for protein expression in mammalian cells - (reply: 2)
  674. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  675. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  676. Amplfy vector - How to amplify vector (reply: 2)
  677. after re-isolation of vector, get single band - (reply: 4)
  678. Cloning big fragment into smaller vector - (reply: 3)
  679. from where can i get the P1 phage - (reply: 1)
  680. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  681. plasmid, BAC, or cosmid? - (reply: 3)
  682. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  683. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  684. Best plasmid miniprep? - (reply: 13)
  685. Plasmid instability in E.coli BL21? - (reply: 3)
  686. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  687. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  688. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  689. Vector sequence included in (PCR) amplified insert - (reply: 2)
  690. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  691. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  692. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  693. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  694. pCLBABE puro retriviral vector - (reply: 1)
  695. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  696. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  697. Plasmid linearization by PCR - (reply: 4)
  698. plasmid sequence identification problem - (reply: 2)
  699. Best lentiviral vector - (reply: 2)
  700. Suitable bacterial reporter vectors - (reply: 3)
  701. plasmid construct problem - (reply: 3)
  702. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  703. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  704. Transient expression vector - (reply: 11)
  705. How to get plasmid maps from publications? - (reply: 5)
  706. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  707. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  708. A gateway cloning expression vector question - (reply: 1)
  709. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  710. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  711. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  712. Low level of expression - in pASK-IBA7 vector (reply: 6)
  713. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  714. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  715. Choice of expression vector - how to choose an expression vector (reply: 1)
  716. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  717. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  718. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  719. Phage removal of E.coli - (reply: 8)
  720. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  721. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  722. Luciferase reporter induced by betagal vector?? - (reply: 1)
  723. PCR problem from transformed TOPO TA vector - (reply: 3)
  724. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  725. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  726. Self-Ligation of digested vector - (reply: 2)
  727. Ligation issue: Does vector size matter? - (reply: 10)
  728. Plasmid sequence - (reply: 1)
  729. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  730. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  731. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  732. What is stringent vector? - Is it related to level of expression? (reply: 2)
  733. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  734. pBMN-GFP as episomal vector - (reply: 1)
  735. where can i ontain the binary vector pCB301? - (reply: 1)
  736. plasmid isolation - (reply: 4)
  737. Plasmid supercoiling affecting PCR? - (reply: 2)
  738. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  739. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  740. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  741. heterodimer - bicistronic vector? (reply: 2)
  742. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  743. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  744. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  745. problem with plasmid extraction - (reply: 3)
  746. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  747. How to transfect two plasmids in a cell - (reply: 9)
  748. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  749. How to separate large size plasmids ~12kb - (reply: 8)
  750. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  751. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 10)
  752. low plasmid yield from midi preps - (reply: 4)
  753. Plasmid isolation, help with dilution step? - (reply: 7)
  754. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  755. full digestion of plasmid to get only dNTPs - (reply: 3)
  756. Lenti viral Vector with GFP - (reply: 4)
  757. inducible shRNA vector system - (reply: 7)
  758. size limit of plasmid origins - (reply: 2)
  759. Creating empty vector by self-ligation - (reply: 31)
  760. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  761. confusion with different forms of plasmid - (reply: 3)
  762. pQE vector protein expression - (reply: 3)
  763. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  764. Plasmid as standard - Plasmid calculation (reply: 6)
  765. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  766. ligation problem with SalI degested vector and insert - (reply: 6)
  767. Effect of EtBr on plasmid supercoiling - (reply: 5)
  768. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  769. lentivirus vector problem--help help - (reply: 6)
  770. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  771. Digestion of a plasmid - (reply: 1)
  772. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  773. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  774. Retroviral vector with luciferase - (reply: 1)
  775. Problems cloning Hairpin vector - (reply: 3)
  776. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  777. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  778. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  779. Amplifying plasmid - non specific binding of primer - (reply: 3)
  780. Storing plasmid at 4 degrees C - (reply: 5)
  781. Program to draw plasmids - draw plasmids (reply: 9)
  782. Transfer of gene from one vector to another vector - (reply: 2)
  783. Transfection of vectors - (reply: 8)
  784. CIP required for double digestion of plasmid? - (reply: 3)
  785. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  786. Plasmid replication - Plasmid replication (reply: 13)
  787. Help with cloning a plasmid that contains repeats! - (reply: 2)
  788. Plasmid extraction sans commercial kits - (reply: 3)
  789. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  790. Myc Tag Vector - (reply: 1)
  791. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  792. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  793. mutations in plasmids - (reply: 3)
  794. Gel purification of vector DNA - low yield - (reply: 26)
  795. Sequential restriction digestion of plasmid - (reply: 58)
  796. Problem cloning oligo into double digested vector - (reply: 9)
  797. Big insert to put back into the vector - (reply: 17)
  798. plasmid spin column usage - (reply: 5)
  799. deleting lacZ gene from control vector - (reply: 5)
  800. Vector for conditional knockout - (reply: 1)
  801. What is the biggest insert for a viral vector? - (reply: 15)
  802. Stored bacteria lost plasmids? - (reply: 12)
  803. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  804. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  805. cloning problems - empty vector (reply: 3)
  806. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  807. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  808. PLASMID ISOLATION - (reply: 10)
  809. Ligation very large vector and small insert - It will not work... (reply: 7)
  810. Frustrated with Plasmid Preps - need help! - (reply: 14)
  811. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  812. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)