HUVEC plasmid transfection challenges - (Jun/26/2013 )

Hi,
I've been trying to transfect primary HUVEC with plasmid DNA but have been running into extremely poor efficiency challenges. I need to do this in 60mm dishes for a subsequent treatment and have tried using Lipofectamine LTX (per manufacturer instructions) with abysmal efficiency.

I can't afford too much cytotoxicity as the cells need to remain strongly adherent to the matrix for the following treatment.

Any help is much appreciated!

-

-cardiovascular-

Lipofectamine (though I haven't tried LTX) in my hands tends to be a lot more toxic and have lower transfection efficiency than fugene. I also quite like PEI.

If you have access to one, an electroporation system may be the best option, they tend to work better with primary cells than lipid/cation based transfection systems.

-bob1-

I found the products of this company very effective with free sample for US:

http://signagen.com/index.php?main_page=index&cPath=137&zenid=ee1b90631232f6752cc2afba4ddbf57b

http://signagen.com/index.php?main_page=product_info&cPath=137&products_id=749

http://signagen.com/index.php?main_page=product_info&cPath=137&products_id=751

http://signagen.com/index.php?main_page=featured_products&disp_order=1&page=2

http://signagen.com/index.php?main_page=specials

http://signagen.com/index.php?main_page=product_info&cPath=137&products_id=753

http://signagen.com/index.php?main_page=product_info&cPath=137&products_id=749

http://signagen.com/index.php?main_page=specials

-BMF-