Plasmid clone in BSP - (Jul/17/2012 )
Hi there,as a newer,there is a question buzz me.It is why there is a plasmid clone of the nested PCR production?Why not directly PCR clone there?Please help me,I will appreciate your kindness!!!
Hope for your answers!
-henry_cheng-
you can directly sequence your pcr products of course. Sequencing after cloning gives your cleaner and more accurate results.
-pcrman-