Plasmid sequencing - (Jan/04/2012 )

I did transformation of a pMIR-REPORT plasmid and when I sequence, I get high background in the sequence. However, there's always 90%+ similarity when I align the sequences. The primers are the ones suggested by the plasmid company and were tested before.

Can anybody help where this comes from?

Thanks in advance.

Cheers,
Bola

This sounds like a sequencing problem. Perhaps you are using either too much or too little DNA in the sequencing reaction. Can we see an .abi file?