very small insert and a very ling vector - (May/26/2011 )
hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody
-mahrak-
mahrak on Thu May 26 11:55:38 2011 said:
hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody
What is tough about it? I would suggest digest - purify - ligate. But maybe I am missing a part here... Could you deliver a bit more details?
-Rsm-