plasmid copy number - method for determination of plasmid copy number (Mar/19/2009 )

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks

somu on Mar 20 2009, 12:13 PM said:

Thank you.