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Staining Related Discussions
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MCF-7 oil red o staining? - (reply: 1)
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Measure intracelluar ROS - Protocol for DCF-DA staining? (reply: 2)
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staining mitotic spindle of leukocytes - a question from a total novice (reply: 3)
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Uneven SYBR green staining/quantitative DNA gels - (reply: 1)
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zymogram of pectinase - activity staining of pectinase (reply: 2)
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romanowsky staining - (reply: 1)
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Do I need to count cells for PI staining? - (reply: 1)
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Using 2 polyclonals (one rabbit, one goat) in double staining? - (reply: 2)
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Protocol for H&E staining for histopathology - (reply: 2)
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microarray reagents for hybridization, wash, stain - (reply: 2)
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How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
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Immunohistochemistry - the best staining? - (reply: 4)
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Immunohistochemistry Even Staining - How to get even staining in IHC (reply: 1)
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staining for receptors in cell line - (reply: 1)
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Is fat cell looked white after HE staining? - (reply: 10)
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Capture of silver stained gels - (reply: 1)
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How to compare the fluorescence staining signal in cells - (reply: 6)
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Silver Staining - Problem in staining (reply: 4)
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TUNEL/PI staining inconsistency - (reply: 1)
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how can I stain a slide like this - (reply: 3)
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Annexin V stain control also, Why? - (reply: 1)
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DNA sequencing - Problem with silver staining (reply: 3)
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Soft agar colony - Problems to stain (reply: 3)
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Some strands in axons are stained with DAPI. - I don't know what they are. (reply: 3)
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Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
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Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
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Immunocytochemistry - staining for cytokeratin - (reply: 5)
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Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
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Any Specific Vital Stains, Dyes? - (reply: 1)
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gold view and sybr safe gel stain - any used gold view gel stain (reply: 2)
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Silver Stain 2D gels - Trouble with silver staining 2D gels (reply: 1)
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nuclear staining without permeablization - (reply: 3)
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Staining of lipid rafts - (reply: 1)
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TRAP staining - TRAP staining of osteoclasts in vitro (reply: 3)
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A few questions on SDS, coomassie staining & western - (reply: 6)
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immunohistochemistry high backgroungd staining - (reply: 6)
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Western Blot Double Stain? - (reply: 4)
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Staining w/ phalloidin and DAPI - Recommendation of staining tissue sections (reply: 5)
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Staining suspension cells for microscopy - (reply: 1)
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CN staining in Western blot? - (reply: 2)
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GUS staining - (reply: 1)
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Spermatozoa staining - (reply: 1)
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Propidium iodide staining of nucleus for IFA - troubleshooting required (reply: 6)
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Dead cell staining on fixed cells - (reply: 1)
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Stain against fungal but not plant material - (reply: 3)
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Are there any methods to replace silver staining? - (reply: 1)
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Arrangement of steps in Gram staining - (reply: 4)
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Questions on Gram staining - (reply: 3)
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SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 3)
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How to detach adherent cells for Annexin V staining in FACScan - (reply: 1)
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peptide - peptide fixation after staining in SDS PAGE (reply: 5)
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Looking for perfect acrylamide gel EtBr stain - (reply: 4)
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Stained Protein Markers - aberrant molecular weights (reply: 3)
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ponceau staining bands - (reply: 1)
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Phosphoprotein staining? - (reply: 5)
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why need both annexin V and propidium iodide staining for apoptosis - (reply: 4)
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CD31 Staining of PFA fixed Frozen Tissue - (reply: 1)
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Use of silver staining for DNA in polyacrylamide gels... - or any other helpful suggestios to improve detection! (reply: 5)
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Is GPF/YFP signal OK after fixation? - Does fixation for immunofluorescent stain cause loss of GPF/YFP signal (reply: 6)
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IHC - No staining whatsoever - (reply: 8)
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problem with the mititracker staining - (reply: 3)
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10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
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Phalloidin staining - problem - (reply: 13)
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Non toxic H&E staining? - (reply: 1)
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Is there ay staining solution to stain colony on agarose gel - (reply: 2)
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Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
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How to send cells for immunfluorescent staining? - (reply: 8)
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How to stain protein fiber to view in light microscopy? - (reply: 1)
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blue evans test - I want to know this staining test, where can I find it? (reply: 1)
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Intense cytoplasmic staining for Hoechst 33258? - not nuclei staining. (reply: 2)
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dead cell staining followed by fixation? - (reply: 4)
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Goldners trichrome staining technique - Why is my bone red and my cartilage green???? (reply: 1)
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Freshness of a culture for staining purposes - (reply: 1)
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Light Microscope-based technique needed - TUNEL staining (reply: 1)
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need a good protocol for actin staining - (reply: 10)
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Apoptosis by PI staining on epithelial cells? - (reply: 3)
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staining cells on transwell inserts - fluorescence microscopy (reply: 3)
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nuclei staining and immunolocalization - (reply: 3)
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In situ hybridization - staining (Fast Red) - Which subforum should we post ISH questions anyway??? (reply: 2)
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SDS-PAGE: Small protein diffuse and not staining well - (reply: 5)
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who can help me with staining with 80kD to 130KD protein family? - it is very difficult for me to successfully staining with integrin (reply: 14)
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Too many problems in 2D - estimation? staining? running? (reply: 4)
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brdu staining on gfp+ve cells - (reply: 1)
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E. coli stain for cloning that grow up very quick - Any one who could give the info??? (reply: 5)
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How to stain MW in nitrocellulose membran? - Western bloting (reply: 7)
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How to eliminate the background staining in the dentin slice - (reply: 6)
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Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
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BrDU staining on GFP positive cells - (reply: 1)
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Fluorescence Stained Sections - Later Examination Possible? (reply: 9)
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Staining polysaccharides in SDS-PAGE - (reply: 3)
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if Annexin-v and PI staining is suitable to attached cells - (reply: 3)
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Is there any good coomassie stain which is comparable to silver stain? - searching for a new staining method for 2D gels (reply: 6)
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Questions about double staining - (reply: 2)
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Problems with DIG DNA labeling! help! - Membrane was completely stained purple with no bands (reply: 12)
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Nuclear staining for 405 nm - (reply: 4)
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Wright-Giemsa staining and phagocytosis assay! - (reply: 2)
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difference between staining vital cell and dead - (reply: 9)
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Can i recover protein from a stained SDS-PAGE? - (reply: 7)
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Amido black staining - (reply: 1)
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No bands after coomassie blue staining - (reply: 1)
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problem with propidium iodide staining - (reply: 1)
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cytoplasm staining - (reply: 5)
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How does PI staining work? - (reply: 3)
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fixing and staining the mammalian colonies with methylene blue for the first tim - could you please show me a protocol (reply: 1)
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cy5 labelt RNA impossible to stain with sybrgreen II ? - (reply: 2)
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DAB staining reagents - (reply: 6)
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adipose stains? - specific stain to positively ID adipose cells (reply: 2)
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FACS PI staining for DNA content analysis - instrumental settings (reply: 1)
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mass spec with silver stain band, unsecssessful. help!1 - (reply: 7)
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intracellular staining : high background and hard to compare MFI - (reply: 3)
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Control for HIF-1alpha staining? - (reply: 1)
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Oil Red O Staining? - Can be done in 6-well plates? (reply: 5)
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Immunofluorescence staining of spheroid cells - (reply: 3)
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Protocol for acridine orange staining of epithelial cells - (reply: 1)
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Identifying structures in a tissue stain - (reply: 1)
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NFkB nuclear staining problems - (reply: 3)
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Silver stain Protein detection - Silver stain problem (reply: 4)
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Silver staining of crystals - To prove it's a complex between 2 proteins (reply: 1)
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Permeabilisation for PI staining in FACS? - (reply: 2)
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showing CPE by Giemsa staining - (reply: 1)
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Does Ponceau stain of NC membrane interfere with Ab binding? - (reply: 4)
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Theory behind coomassie stain/destain? - Does adding tissue to the destain really speed destaining? (reply: 7)
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laser capture microdissection-tissue staining protocol - (reply: 2)
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Double staining antibody plus Annexin! - Help (reply: 2)
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nuclear stain for living cell. any recommendation for dye? - (reply: 6)
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Toto-3 staining - (reply: 2)
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Trouble staining for Fluorescent Microscopy - unspecific labeling (reply: 2)
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three color staining - (reply: 3)
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RNA stain on blot? - is there a way to see the total RNA on a blot? (reply: 1)
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the dilute concentration for DAPI staining - (reply: 17)
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Silver staining after Coomassie stain - (reply: 7)
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Streptavidin-biotin peptide pull down for silver stain - (reply: 2)
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About to run FACS: But I forgot my single stain controls! - (reply: 1)
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dna staining dyes - which DNA staining dyes do you use (reply: 4)
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PMA/ionomycine activated cells - intracellular staining after saponin permeabilisation (reply: 4)
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alternative DNA staining dyes - which DNA staining dyes do you use (reply: 6)
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IHC staining - (reply: 1)
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CV staining rat brains - (reply: 5)
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Blotting gel after Bio-Rad Coomassie staining? - (reply: 5)
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Silver Staining Principle and protocol - (reply: 2)
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stain genomic and plasmid DNA - FACS analysis (reply: 1)
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ponceau red staining solution - do you have a prefered one? (reply: 4)
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destaining silver stained gels - (reply: 1)
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Gel Electrophoresis & Staining - (reply: 2)
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silver stain can even get band with the loading buffer control - (reply: 4)
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Flow Cytometry Intracellular and dead cell staining - (reply: 2)
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PSD95 staining - (reply: 5)
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help chosing a fluorescent DNA stain - (reply: 3)
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reversible stain? - (reply: 4)
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PI staining of adherent cells in plates for FACS - can this be performed in plates? (reply: 3)
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temperature of silver staining - (reply: 3)
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collagen H&E staining - tissue collagen staining (reply: 3)
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collagen staining mason's - Mason's Trichrome stain -collagen staining (reply: 1)
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Help: Can't see any positive staining at all... SDS PAGE gel - (reply: 5)
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Fibroblast staining - (reply: 2)
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Staining of Gels - which is the best (reply: 2)
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stain similar to neutral red - stain unfixed cells directly (reply: 4)
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PI staining - (reply: 11)
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HISTOLOGY HELP! - Identifying a stain - (reply: 8)
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cell stain, nonfluroescent - (reply: 4)
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Silver stain vs Western blot - (reply: 5)
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Problems with AnnexinV-FITC staining - (reply: 2)
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MCF-7 staining protocol - nile red, or oil red o? (reply: 2)
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cells stained with NF-KB and DAPI - (reply: 11)
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BODIPY-FL-ceramide from invitrogen for Golgi staining - Question on staining protocol (reply: 2)
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Immunofluorescent staining controls - (reply: 2)
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Which method of RNA staining is less costly - (reply: 3)
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BrdU incorporation and GFP staining - (reply: 3)
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Problem with X gal staining - (reply: 1)
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silver staining trouble - (reply: 3)
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method for staining of invisible cell pellets? - (reply: 1)
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some IHC staining problems - (reply: 3)
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protocol for Hoescht Staining - (reply: 4)
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Nuclear staining instead of cytoskeleton - (reply: 12)
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Need protocol: CFSE Staining/ primary cells - (reply: 2)
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staining the gel - (reply: 5)
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Imaging cells in matrigel - how to stain ? Prepare ? (reply: 1)
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cell stain and the fixation? - (reply: 2)
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silver nitrate staining - (reply: 1)
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NF-kB staining failed - (reply: 1)
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Staining picogram quantities of short, single-strand DNA - (reply: 6)
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Tryptic digest help - Trouble diffusing stain (reply: 3)
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stain for fungus - (reply: 1)
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Dsitinct Bands on Ponceau Stain After Sonication? - (reply: 4)
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puncta staining problem - (reply: 5)
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Silver Staining Protocol for DNA - EDTA solution concentration missing (reply: 1)
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Annexin staining - strange & mysterious population - (reply: 3)
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salvaging silver staining mistake? - (reply: 1)
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HOW TRYPHAN BLUE STAINING WORKS? - (reply: 1)
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two mouse antibodies can be stained with different secondaries? - different colors? (reply: 3)
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Time between induction of apoptosis and use of AnnexinV staining - (reply: 3)
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Von Willebrand staining - (reply: 1)
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x-gal staining of embryos (whole mount) - possible after methanol? (reply: 1)
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Silver Staining - (reply: 2)
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(Alcian Blue) Staining Solution Preparation - (reply: 1)
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How to reduce the background of Annexin V staining? - (reply: 6)
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Methylene blue staining and photometry question - (reply: 4)
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Tryphan Blue staining for cell viability - (reply: 1)
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Silver Staining - (reply: 7)
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Nuclear staining - staining with a non-nuclear antibody - (reply: 1)
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Stains for proteins.. - Silver v/s Coomassie (reply: 1)
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Multiple unspecifc bands in IP using Protein A sepharose + silver staining - (reply: 2)
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How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
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SYBRSAFE staining - (reply: 2)
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How to stain native proteins on PVDF before immunoblotting - (reply: 3)
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SDS-PAGE Staining - (reply: 5)
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the step for staining bacteria cell wall - (reply: 1)
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Silver Staining - (reply: 1)
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staining - Amido black staining (reply: 3)
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Problems with BrdU staining - (reply: 3)
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Non-specific binding of secondary antibodies in IF - Secondaries stain Swiss 3T3 nucleoli (reply: 1)
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Different trend of results between silver staining and coomassie blue staining? - (reply: 9)
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How amido black stains protein? - (reply: 1)
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Marker for plasma cell staining - (reply: 1)
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Ethidium bromide - how to throw ethedium bromide after staining (reply: 4)
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Double immunofluorescence staining (cyto) - anti-mouse and anti-rat, too similar? (reply: 4)
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Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
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endothelial cells' staining - (reply: 7)
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DNA stains for fluorescence spectroscopy. - (reply: 4)
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Alizarin red/Trypan blue stain - (reply: 3)
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AFLP gel staining trouble - (reply: 1)
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Staining Bacteria - There is a wide variety of stains, does it matter which one I use? (reply: 3)
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Ethidium Bromide vs Sybr Green stain (normal PCR) - (reply: 5)
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Staining Brains (immunofluorescence) - Any tips to improve staining? (reply: 4)
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two color in immunoperoxidase staining - (reply: 5)
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where can I find protocols for nucleus staining? - (reply: 1)
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Amidoblack staining - (reply: 1)
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How to stain /destain quickly SDS-PAGE gel? - your patents? (reply: 13)
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ICC: High fluorescence cell body staining - secondary staining all neuron cell bodies (reply: 7)
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help for bacteria endospores fluorescent stain - (reply: 1)
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PVDF staining problem... - (reply: 3)
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DNA stain for viable FACS cell sorting? - (reply: 7)
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Staining SDS-PAGE prior to transfer - (reply: 1)
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How to avoid getting a precipitate with Giemsa staining??? - (reply: 2)
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FACS control high background - more stained cells in control than in sample (reply: 4)
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Ladder for silver staining - (reply: 2)
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silver staining prob - (reply: 3)
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Tissue staining problem with polyclonal antibody - (reply: 3)
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staining gels and western blot - (reply: 1)
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Gram stain basic question - (reply: 2)
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Is coverslip needed after Gram stain? - (reply: 7)
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Eliminating background staining for ICC - ICC (reply: 1)
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Fixing in staining - (reply: 1)
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Staining HT29 cells with TLR Abs and FITC, TRITC and DAPI - (reply: 6)
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Problem with phalloidin staining - (reply: 2)
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Fixation method for phalloidin staining - (reply: 3)
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DAPI staining - (reply: 2)
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Apoptosis - how to rduce the the background staining for annexin V - (reply: 1)
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Help! Non-specific staining with ICC - (reply: 2)
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Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
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Nauta staining for axonal degeneration - (reply: 1)
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Non-specific staining with secondary... - Scott are u there?? (reply: 6)
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Coomassie Blue stain keeps precipitating - (reply: 2)
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Help, problems with silver staining - (reply: 2)
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Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
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has anybody done live cell staining? - (reply: 6)
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A question about cell stain: fix the cell have fusion protein - (reply: 1)
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gel staining - (reply: 1)
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background staining in immunofluorescence - (reply: 6)
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Silver staining and MALDI MS - can they work together? - silver stained 2DE spots = no MALDI MS results (reply: 2)
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ethidium bromide staining of DNA gels - what's a good alternative to EtBr (reply: 8)
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sensitivity and staining specificity of sypro vs. proQ - (reply: 1)
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coomassie blue stained gel - possible to obtain good blotting onto membrane? (reply: 3)
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Immunofluoroscence (IF) staining problem - problem in NF-kB staining (reply: 5)
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B-gal staining of cells - (reply: 1)
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Alternative stains for DNA - Alternative stains for DNA (reply: 2)
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more sensitive than silver staining - (reply: 1)
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Non toxic gel staining for highschool student work - Agarose gel stains (reply: 3)
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silver staining - (reply: 1)
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Problem in sliver staining - (reply: 2)
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Immunohistochemistry - no positive staining - (reply: 1)
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Eletrophoresis - Ab caspasis 3 staining (reply: 1)
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Western blot - Pounceau S staining problem - (reply: 16)
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gel stain and gel extraction - crystal violet (reply: 1)
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B-gal staining on fixed samples? - (reply: 2)
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Staining colonies in soft agar... - (reply: 1)
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Staining colonies in soft agar - how?? need fixation?? (reply: 1)
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Has any body tried the EMSA with Silver Staining - (reply: 1)
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Staining colonies in soft agar - (reply: 1)
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immunohistochemistry protocol - problems with staining (reply: 1)
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Haematoxylin Eosin staining for frozen sections - H&E staining frozen sections (reply: 4)
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Help! Cell staining problem - (reply: 3)
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Strange mobility behavior of PCR products on agarose gel - EB stained vs Gelstar stained (reply: 2)
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cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
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Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
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background staining in IHC ofartificial oralmucosa - (reply: 2)
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DAPI in mammalian cells - bright spots in DAPI staining (reply: 1)
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Cell staining - (reply: 1)
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desmosomes - antibody which stains desmosomes! (reply: 2)
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mesenchymal stem cells - fixation for staining (reply: 6)
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Double staining protocols for Insitu hybridization and immunohistochem - (reply: 1)
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Cell wall stain - (reply: 1)
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Staining and destaining PAGE gels - Alternative method for stain and destain (reply: 4)
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CFSE staining - (reply: 1)
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Insitu antibody staining - (reply: 1)
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Double Staining - (reply: 1)
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degraded cDNA from immunohistochemically stained tissues - degraded cDNA from immunohistochemically stained tissues (reply: 1)
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Polymyxin B conjugate stain - (reply: 3)