Intense cytoplasmic staining for Hoechst 33258? - not nuclei staining. (Dec/18/2007 )

Hi, all,

I got this weird staining pattern for Hoechst dye for MCF-7 cells. I was trying to stain the nuclei, but I got mostly cytoplasm. Any ideas?

-genehunter-1-

QUOTE (genehunter-1 @ Dec 18 2007, 10:46 AM)

Hi, all,

I got this weird staining pattern for Hoechst dye for MCF-7 cells. I was trying to stain the nuclei, but I got mostly cytoplasm. Any ideas?

Could it possibly be internalised MYCOPLASMA contamination..........I do hope not as I am sure you regularly mycoplama test your cells?

-Rhombus-

I recent got this line from the other group. It looks like suspicious to me. Thanks.

-genehunter-1-