silver staining prob - (Aug/25/2005 )

hi
i have been trying to do silver staining in my lab.. m the only one who does it, so have nothin else to fall back on except printed protocols
the thing is that the 1st time i tried it, it worked wonderfully (i used the DTT, glutaraldehyde method)
but since then, i have tried reproducing ot, but it just doent work!
do i have to prepare fresh solutions every time i plan to do a silver staining?
i prepare AgNO3 n the developing solution fresh everytime.......
can anyone help?

Hi
I have done silver staining few times with Proteosilver plus Silver stain kit from Sigma and it works well.. just follow the protocol exactly..
All the best!

thanx!
ill defenitely let u know abt the results!

this method has worked flawlessly for me:
http://pcl.tamu.edu/

(under staining protocols on left-hand side of web page)