Protocol Online logo
Top : Forum Archives: : Immunology and Histology

cytoplasm staining - (Aug/01/2007 )

Does anyone know how to stain cytoplasm of live cells?

-minnee-

Is staining for cytoskeletons close enough?

-genehunter-1-

Will GFP help ?

-scolix-

Actually I was looking for some dye to stain cell cytoplasm.

-minnee-

QUOTE (minnee @ Aug 1 2007, 07:21 PM)
Actually I was looking for some dye to stain cell cytoplasm.


use calcein;

GFP alone may also stain perinuclear vesicles/cisternae

-The Bearer-

hi

may you want to try Eosin or difquick I solutions for staining cytoplasm portion (but we use only for fixed cells).

gud luck

QUOTE (minnee @ Aug 1 2007, 05:35 PM)
Does anyone know how to stain cytoplasm of live cells?

-payeli-