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Immunohistochemistry - the best staining? - (Oct/16/2008 )

hai,

I have to do the immunohistochemistry of frozen tissue samples. These tissue samples are from virus infected chickens. i am confused as to which system to use for detection of viral antigens - either ImmunoFIuroscence or Peroxidase system. As i can see in the recent journal articles mostly peroxidase system has been employed, Experts in this field, please give me your views.

thanks in advance

-paapu-

I would use peroxidase system.


-Minnie Mouse-

QUOTE (Minnie Mouse @ Oct 20 2008, 05:20 PM)
I would use peroxidase system.


any specific reason for choosing peroxidase?

-paapu-

I would use whatever you have onhand or is available in the lab.
Personally I really like immunofluorescence, but I do a lot of combination stainings, so it is dependend on what you're looking at/for...
Also if there people around that have done either one that you know, just ask them I'm sure they can help you.

-Ddkb-

QUOTE (Ddkb @ Oct 29 2008, 03:25 PM)
I would use whatever you have onhand or is available in the lab.
Personally I really like immunofluorescence, but I do a lot of combination stainings, so it is dependend on what you're looking at/for...
Also if there people around that have done either one that you know, just ask them I'm sure they can help you.


thank u very much

-paapu-