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91. bacterial cultures - growing to saturation? (reply: 4)
92. purging with inert gas? - (reply: 1)
93. PBS with and without calcium/Magnesium - Ca is good for you...is it good for PBS? (reply: 1)
94. Sensitivity of Griess Reagent - How could they do it!? (reply: 8)
95. wobbly bands in western blotting - (reply: 5)
96. decontamination of ethidium bromide - (reply: 1)
97. cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
98. Looking for nucleotide sequence of rearranged antibody gene locus. - (reply: 1)
99. adenovirus CsCl centrifugation - (reply: 2)
100. Does anyone know how to prepare ammonium molybdate solution? - (reply: 1)
101. When to use PMSF in a cell lysis buffer? - (reply: 9)
102. staining for receptors in cell line - (reply: 1)
103. Solubility assessment - (reply: 1)
104. PCR negative control contaminated and not the other wells - (reply: 4)
105. Counting B cells in hemacytometer - (reply: 2)
106. Hemocytometer Measurements - (reply: 1)
107. Inoculating Loop Handling - (reply: 1)
108. Expired chemicals - (reply: 2)
109. TBE gel, in TAE buffer. - (reply: 2)
110. Where can I buy portable bunsen burners? - (reply: 4)
111. How much loading dye? - (reply: 4)
112. How much salt can/should be used for DNA precipitation? - Is there a lower or upper limit? (reply: 8)
113. Agrose gel - (reply: 4)
114. Elimininating RNAses - (reply: 3)
115. Resulting pH of two salts - (reply: 1)
116. GUS Staining - buffer (reply: 2)
117. Radioenzymatc Assay - How to convert cpm values into enzyme specific activity (reply: 1)
118. TAT protein - (reply: 1)
119. PEG didn't precipitate right away....what is your experience or advice? - PEG precipitation (reply: 8)
120. Substituting buffering agents - Did I go to far? (reply: 1)