Solubility assessment - (Oct/13/2008 )

Hi everyone,

i have to test a few compounds in vitro and the first step is to do a solubility assessment (using water or DMSO).

What it's the proper way to do it? just adding compound until I reach the maximum concentration of 5000microg/ml??

Thanks!!

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if the properties of the substance is unknown, you may know the chemical class to decide if it is more hydrophilic or not; then you can take data from literature to get an idea;

if the substance is totally unknown, you have to do as suggested; warming and vigorously shaking may help; beside water and DMSO, methanol may also be checked...

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