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Tissue Related Discussions
  1. Protocol for RNA preparation from Formalin Fixed tissues - (reply: 3)
  2. cell and tissue culture - healthy test (reply: 1)
  3. Homogenizing buffer for mouse liver tissue - (reply: 4)
  4. How to release Ca2+ from calcified tissue? - (reply: 3)
  5. Troubles in IL-8 measurement of tissue cells cultured with E.coli - (reply: 1)
  6. FW: Sandwich ELISA with brain tissue homogenates - Question asked by Natasa (reply: 3)
  7. Brain tissue homogenates and IL-1 - (reply: 1)
  8. general protein purification from mouse tissue - (reply: 4)
  9. Best storage temperature for samples - serum, protein, tissue, DNA, RNA... (reply: 5)
  10. tissue "fixation" - (reply: 1)
  11. protein extraction from mouse brain tissue - (reply: 1)
  12. Cryosectioning skeletal muscle - I have huge problems with tissue damage (reply: 3)
  13. total protein extraction from brain tissue - (reply: 3)
  14. processing of tissue for ELISA - (reply: 3)
  15. housekeeping genes for brain tissue - (reply: 4)
  16. housekeeping genes for brain tissue - (reply: 3)
  17. Strawberry Tissue culture - (reply: 1)
  18. Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
  19. harvest DNA or RNA from paraffinembedded tissue? - Is it possible to use a block of tissue for other analysis than IHC? (reply: 4)
  20. Total RNA from mouse tissue - (reply: 3)
  21. how do u determine actual concentration from absorbance readings? - using tissue lysates for ELISA sample (reply: 3)
  22. tissue flask - (reply: 2)
  23. Immunohistochemistry in human skin tissue - (reply: 1)
  24. where can i purchase human tissue samples? i.e. adipose tissue, liver, muscle - (reply: 3)
  25. How long tissue can stary in sucrose or fixative before cryosectioning? - (reply: 5)
  26. tobacco tissue culture - (reply: 2)
  27. my tissue sections are very fragile ! - (reply: 5)
  28. Staining w/ phalloidin and DAPI - Recommendation of staining tissue sections (reply: 5)
  29. Why does DMSO turn tissue black? - (reply: 1)
  30. Fresh tissue slicing - need to fix in two different fixatives (reply: 1)
  31. Immunofluorescence on mouse tissues - (reply: 2)
  32. Protein isolation from RNAlater stored tissue - (reply: 5)
  33. RNA extraction from skin tissue - (reply: 2)
  34. How to differ macrophage from the other in tissue? - (reply: 3)
  35. who can give advice about larger sample number tissue crushing - methods/machines that can help w breaking of dozens of tissue samples (reply: 6)
  36. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  37. Isolating RNA from frozen tissues kept in vials - (reply: 4)
  38. CHIP : Use frozen tissue - (reply: 1)
  39. 3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
  40. PCR with paraffin-embedded tissue DNA samples - I have had problems to amplify DNA samples from paraffin-embedded tiss (reply: 1)
  41. SNPs: blood and tissue - (reply: 1)
  42. how to check organ(tissue)-specific expression profile of a gene - (reply: 3)
  43. housekeeping gene for comparing gene expression between tumor and normal tissue - (reply: 1)
  44. DNA extraction from dried tissue - (reply: 1)
  45. Nasal Mucosa tissue coming off slides - (reply: 3)
  46. Protocols for Preparation of cell lysate from tissue sample - (reply: 3)
  47. What are the benefits to extract DNA from tissues such as liver, heart and kidne - (reply: 5)
  48. Nuclear extraction of brain tissue for ChIP - (reply: 3)
  49. Tissue Sectioning Question - (reply: 3)
  50. how cells adhere to the surface of tissue culture flaask? - basic question (reply: 3)
  51. Problems loading equal amounts of tissue lysate - This is driving me nuts!!!!! (reply: 1)
  52. Problems with Western blotting of tissue homogenates - (reply: 3)
  53. CD31 Staining of PFA fixed Frozen Tissue - (reply: 1)
  54. Storing protein extracted from tissues for Western blot - (reply: 9)
  55. Storing tissue for protein and RNA extraction? - (reply: 13)
  56. Isolation of RNA from Difficult Tissues - (reply: 3)
  57. Genomic DNA Extraction - Frozen Tissue (reply: 5)
  58. Custom plasticware for tissue culture - (reply: 3)
  59. How to Lyse Adipose Tissue - (reply: 2)
  60. help with identifying deletions in tissues - (reply: 3)
  61. looking for TNF alpha antibody for human tissue IHC - (reply: 1)
  62. Cell isolation from tissue -- how do you tell how many viable cells you get? - (reply: 3)
  63. Membrane protein extraction from mice tissue - (reply: 3)
  64. protocol for: milling of tissue with ball mills - looking for a automation to isolate "living" bacteria out of (reply: 3)
  65. Snap freezing tissue for RNA isolation - (reply: 10)
  66. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
  67. realtime from different starting tissue masses - (reply: 1)
  68. How to know tissue specific gene expression by real time PCR - (reply: 3)
  69. Question regarding muscle tissue homogenization - Why rotate homogenates after homogenization? (reply: 2)
  70. Immunohistochemistry of anti-ki-67 on paraformaldehyde-fixed mouse frozen tissue - (reply: 2)
  71. Gluteraldehyde fixation on tissue - How long is too long? - (reply: 2)
  72. How much serum should I put in the tissue culture - (reply: 5)
  73. Activation of JNK signaling pathway using frozen liver tissue? - (reply: 3)
  74. please help: how to isolate nuclear protein from tissue samples - (reply: 1)
  75. Gel shift on formalin tissues? - EMSA (reply: 1)
  76. Who makes 200-350cm2 tissue culture roller bottles? - (reply: 8)
  77. Imprinted gene control in breast tissue? - (reply: 1)
  78. Tissue culturing - Crash down of culture -Unknown reason!! (reply: 4)
  79. Mouse Ab on Mouse Tissue: Blocking Problem - (reply: 2)
  80. microbial contaminant in plant tissue culture...?! - (reply: 1)
  81. DNA extraction - starting from mouse ear tissue (reply: 1)
  82. Tissue expression database? - Looking for a web page.. (reply: 1)
  83. OPINIONS on Plant Tissue Culture- - Please post your opinions and views on plant tissue culture. (reply: 1)
  84. After extracting total RNA from tissue using trizol reagent and phase lock gel,h - I am going to extract bothe total RNA and genome DNA from one sample (reply: 4)
  85. Isolation of total RNA from different tissues - efficiency? - (reply: 2)
  86. please help in Anthurium & Orchid Tissue culture - tissue culture medium (reply: 2)
  87. human dermal fibroblasts tissue culture protocol - (reply: 2)
  88. thawing frozen tissue and culturing - (reply: 1)
  89. Need clean HAtag antibody for mouse tissue lysates - (reply: 1)
  90. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
  91. Agar mixture for plant tissue - (reply: 9)
  92. tissue on slides - tissue on slides (reply: 3)
  93. Co-IP from tissues - Co-IP protocol for tissues (reply: 5)
  94. tissue preparation for human/mouse islet cells - (reply: 1)
  95. Protein from Liver tissue - (reply: 2)
  96. problem in DNA extraction from glycerol-ethanol embedded tissue - (reply: 1)
  97. does fixation of frozen tissue sections preserve protein-protein interactions - immunohistochemistry (reply: 1)
  98. Why not dehydrate tissue in methanol before paraffin embedding? - (reply: 1)
  99. Bisulfite modification of DNA from paraffin embedded tissue - (reply: 4)
  100. isolation of celmembrane of tissue cells - (reply: 2)
  101. live imaging on slice tissue - (reply: 3)
  102. Total protein extraction from Leaf tissue - (reply: 1)
  103. Microplate Bradford with Tissue Whole Cell lysate - (reply: 1)
  104. lung tissue homogenization - (reply: 1)
  105. Identifying structures in a tissue stain - (reply: 1)
  106. Tissue Homogenization for Lactic Acid Level - (reply: 5)
  107. Difficulty Isoalting DNA From Adipose Tissue - (reply: 1)
  108. Identifying structures in tissue stain - Stain of the human tongue (reply: 2)
  109. About the adenovirus infection efficiency on tissue culture - (reply: 1)
  110. Rat Brain Tissue RNA Isolation with TRIzol - (reply: 9)
  111. Protocols for RNA extraction from 5-10mg starting tissue samples - Molecular Biology (reply: 1)
  112. ChIP in mouse tissues, How to crosslink ? - (reply: 1)
  113. Theory behind coomassie stain/destain? - Does adding tissue to the destain really speed destaining? (reply: 7)
  114. To compare normal and cancer tissue - (reply: 5)
  115. tissue processing - microtome (reply: 1)
  116. RNA isolation from mouse heart tissue - (reply: 2)
  117. Duration of Maximum Formalin fixing for tissue - (reply: 1)
  118. Duration of Maximum Formalin fixing for tissue - Formalin fixing for tissue (reply: 1)
  119. DNA Methylation - Tissue specific - (reply: 4)
  120. How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (reply: 2)
  121. Translation of mRNA in particular tissue - (reply: 2)
  122. Tissue spots shred from Tissue Microarray Slide - (reply: 3)
  123. Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
  124. how larg should my tissue sample be for western blotting? - (reply: 1)
  125. Studying cell metabolism from frozen tissue samples - Is this even possible? - (reply: 2)
  126. DNA from slide mounted epoxy embedded fixed tissue - (reply: 1)
  127. Contamination rate high! - tissue culture (reply: 4)
  128. RNA extraction from TissueTek embedded tissue - (reply: 1)
  129. DNA from somatic tissues for bisulfite treatment - (reply: 3)
  130. Establishing cell lines from brain tumor tissue samples - (reply: 2)
  131. immunohistochemistry protocol for rats tissue - protocol for immunohistochemistry using rat tissue (reply: 1)
  132. how to ship tissue slides? - (reply: 1)
  133. RNA extraction of a very, very small tissue sample - (reply: 2)
  134. Obtaining cells from human tissue samples - (reply: 2)
  135. looking for a protocol for in vitro treatment of tissues - (reply: 2)
  136. RNA extraction/troubleshooting - specifically for older tissues (reply: 2)
  137. preservation of tissues for later on RNA isolation. - (reply: 3)
  138. How to normalize the protein expression amongst different tissues - (reply: 7)
  139. How to isolate RNA from zebrafish tissue? - (reply: 1)
  140. WB and tissue samples - (reply: 2)
  141. ChIP on frozen tissue samples - (reply: 7)
  142. what tissue is "least nervous"? - (reply: 11)
  143. Obtaining single cell suspension from fixed biopsy tissue - (reply: 2)
  144. PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
  145. contamination of plant tissue culture - (reply: 2)
  146. Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
  147. gDNA salvage for PCR from mounted tissue sections - Is it possible? (reply: 1)
  148. animal tissue extraction? - (reply: 1)
  149. How to avoid contamination in tissue culture? - (reply: 11)
  150. Tissue Culture of Sealing Red Wax Palm - (reply: 3)
  151. anyone worked with fish tissue culture? - (reply: 1)
  152. Total RNA from archival tissue that is fixed and paraffin embedded (like brain t - (reply: 1)
  153. Cell Lines from Tumor Tissue Samples - Advice/Protocols on best techniques (reply: 2)
  154. tissue n cell culture - (reply: 3)
  155. frozen tissue prep for IHC and westerns - (reply: 1)
  156. Tissue Culture - (reply: 1)
  157. How to detect micRNA in some certain tissue ？ - (reply: 4)
  158. RNA extraction from muscle tissue using RNeasy kit - Can I merge these two protocols together? (reply: 2)
  159. Shearing of genomic DNA - Shearing of genomic DNA from prawn tissues(Gills, Muscle, Hepatopancreas and Eye (reply: 8)
  160. DNA extraction from formalin-fixed tissue - (reply: 2)
  161. Maize tissue culture - (reply: 5)
  162. is tissue from rat kidney reacting with ECL? - (reply: 9)
  163. iNOS and nNOS protein quantification from rat tissue homogenate - (reply: 1)
  164. desert plants - for tissue culture (reply: 6)
  165. Slices from frozen brain tissue - (reply: 1)
  166. best kit for RNA extraction from animal tissue - (reply: 3)
  167. protein extraction from tissue - (reply: 1)
  168. somaclonal variation in tissue culture - (reply: 4)
  169. Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
  170. MSP for formalin-fixed and paraffin-embedded tissues - (reply: 2)
  171. Tissue culture Infective dose - Infect the Human tumor ell culture by Newcastle virus (reply: 1)
  172. When air bubbles is not encouraged in tissue culture? - (reply: 4)
  173. arabidopsis leaf tissue culture - (reply: 3)
  174. Tissue sample storage - (reply: 1)
  175. RNA isolation from frozen tissue sections - Which Kit do YOU use? (reply: 3)
  176. RNA from plant tissue shows 3 rRNA bands - RNA isolation by Trizol method (reply: 2)
  177. DNA / RNA extraction from freeze dried tissue - (reply: 6)
  178. single cells from human tissue? - need protocols (reply: 1)
  179. Plant tissue culture - (reply: 1)
  180. Plant tissue culture - (reply: 7)
  181. extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
  182. Smearing of protein samples from whole tissue lysates - (reply: 6)
  183. Plant tissue culture - (reply: 4)
  184. RNA Isolation from Breat Normal tissue - (reply: 3)
  185. western blotting of tissues - (reply: 1)
  186. Tissue transfer - Tissue transfer (reply: 2)
  187. RNA degredation - Extracting RNA from Brain Tissue (reply: 8)
  188. Poor 260/280 ratio for genomic DNA from tissue - (reply: 1)
  189. Liver tissue lysis for ER protein Western blotting - (reply: 2)
  190. Several questions about Western blotting of tissues - (reply: 2)
  191. how to determine tissue specific expression of gene? - (reply: 2)
  192. Problem amplifying DNA from tissue - (reply: 4)
  193. RNA isolation from tissues: RNA pellet is yellow-brown? - Why this? (reply: 2)
  194. Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
  195. Problem with genomic DNA extracted from mammalian tissue using PCI method - (reply: 1)
  196. ChIP analysis using tissue and controls - (reply: 3)
  197. how to shear tissue DNA - (reply: 2)
  198. Down-regulation of house-keeping genes in colon tissue of DSS-fed mice - (reply: 1)
  199. tissue culture about zizyphs jujuba - please help me! (reply: 2)
  200. Taqman for low levels of a gene (LacZ gene)in tissue - (reply: 1)
  201. tissue culture Garlic/Onion - protocol (reply: 4)
  202. Primary culture of breast tumour - Does anyone know the tissue slice method? (reply: 3)
  203. DNA extraction from formalin fixed tissue - (reply: 1)
  204. RNAlater - how to handle tissue stored in RNAlater (reply: 1)
  205. protein isolation of plant tissue -trizol regent - (reply: 3)
  206. activity measurements on fixed tissues - (reply: 1)
  207. Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
  208. Tissue culture -Tips - (reply: 6)
  209. help for Isolation of FIBROBLAST from tumor(tissue speciment) - (reply: 2)
  210. ideas n opinions plant organelle - frozen plant tissue organelle isolation (reply: 1)
  211. need help on learning cell and tissue culturing - (reply: 6)
  212. Natural tissue affinity of AEC used in IHC - AEC is a better substrate in immunoperoxidase test (reply: 1)
  213. Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
  214. Non CpG cytosine methylation - Global methylation analysis of somatic tissue (reply: 1)
  215. Tissue staining problem with polyclonal antibody - (reply: 3)
  216. Is 100 ug liver tissue protein lysate too much for western blot? - (reply: 2)
  217. Formalin-fixed tissue - DNA extraction - (reply: 3)
  218. RNA extraction from nitrogen-frozen tissue - (reply: 2)
  219. Bacterial DNA extraction from plant tissues - (reply: 1)
  220. Lysis buffers for brain tissue samples - Which ingredients are superfluous? (reply: 3)
  221. Source for PAR antibody? - IHC for Poly-ADP-Ribose on rat tissues (reply: 2)
  222. Can ChIP be applied on tissue samples? - (reply: 5)
  223. TIssue Homogenization Buffer W/o DTT - Missing Reagent (reply: 1)
  224. plant tissue culture protocols - basic plant tissue culture techniques for students (reply: 8)
  225. homgenize and lyse the tissue together with sonication - feasible ? Why not? any comments? (reply: 3)
  226. Protein extraction from annatto fruit tissues - (reply: 3)
  227. Tissue culture of algea - (reply: 3)
  228. Tissue prepatation for WB and other issues - (reply: 5)
  229. Tissue storage for RNA isolation - (reply: 4)
  230. Expected DNA extraction yield from tissue - (reply: 1)
  231. Eyes of a tissue culturer - Needed!! see this!! (reply: 3)
  232. Tissue and cDNA microarray? - (reply: 1)
  233. tissue ChIP - (reply: 2)
  234. Can I use the upstate ChIP protocol for mouce tissue? - (reply: 5)
  235. Isolation of total RNA and mRNA from human tissue - (reply: 5)
  236. immunohistochemistry - tissue won't stay on slides - (reply: 5)
  237. Tissue culture and cell culture? - (reply: 4)
  238. Protein extraction from rat liver tissue - (reply: 1)
  239. bands from tissue one size, from cells - size doubles - help! (reply: 4)
  240. DNA extraction method for alcohol preserved plant tissues - (reply: 1)
  241. RNA Extraction using Trizol - How much Trizol should I use for rat brain tissue (reply: 5)
  242. DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
  243. FACS analysis for tissue samples? - (reply: 2)
  244. check protein expression from tissue - (reply: 1)
  245. Anti-Flag M2 crossactivates with mouse tissue? - (reply: 1)
  246. RNA extraction - RNA from 20yr old frozen breast tissue (reply: 1)
  247. DNA RNA extraction from adipose tissue - (reply: 2)
  248. Proteinase digestion of tissue for DNA extraction - (reply: 3)
  249. tissue culture of jatropha - tissue culture of biodiesel producing plant (reply: 22)
  250. Formalin Fixed Tissue westerns - (reply: 1)
  251. breast tissue disaggregation - (reply: 1)
  252. tissue culture floor cleaning - standard protocol?? (reply: 1)
  253. tissue homogenization - Xenopus kidney homogenization (reply: 1)
  254. connective tissue autofluorescence - (reply: 1)
  255. RNA Extraction from ocular tissue - RNA Extraction from ocular tissue (reply: 2)
  256. Can RNA be extracted from paraffin-fixed tissues? - RNA extraction protocol for paraffin (reply: 1)
  257. tissus proteins extraction - (reply: 3)
  258. How to get enough cell alivefrom colon cancer tissue? - (reply: 2)
  259. Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
  260. Monoclonal antibodies on MOUSE Tissue - Monoclonal antibodies on MOUSE Tissue (reply: 2)
  261. degraded cDNA from immunohistochemically stained tissues - degraded cDNA from immunohistochemically stained tissues (reply: 1)
  262. DNA extraction from formalinfixed tissue - (reply: 2)
  263. detection of calpain activity inthe Red blood cell and other tissues - (reply: 1)
  264. How perform RNA extraction fromformalin-fixed tissues?? - (reply: 1)
  265. Isolation of total RNA from animal tissues and cells - by GuSCN and silico (reply: 1)
  266. Lyophilized or Silica gel dried tissue protocols - DNA protocols (reply: 1)
  267. ELISA for frozen fat tissue - (reply: 1)
  268. Re: How to extract RNA fromFormalin fixed tissue - (reply: 7)
  269. DNA/RNA extraction from formalin fixed tissues - (reply: 1)