How to Lyse Adipose Tissue - (Jan/30/2008 )

Hi there,
I would really appreciate any protocol for the lysis of frozen adi[ose tissue....
for western Blot

Thanks,

-Zhenya-

QUOTE (Zhenya @ Jan 30 2008, 08:09 AM)

Hi there,
I would really appreciate any protocol for the lysis of frozen adi[ose tissue....
for western Blot

Thanks,

Here is a general protocol on lysing primary cells from tissue to obtain protein for Western blots:
http://biologicalworld.com/lyseprimarycells.htm

-keithbrent2000-

QUOTE (keithbrent2000 @ Feb 3 2008, 09:31 AM)

QUOTE (Zhenya @ Jan 30 2008, 08:09 AM)

Hi there,
I would really appreciate any protocol for the lysis of frozen adi[ose tissue....
for western Blot

Thanks,

Here is a general protocol on lysing primary cells from tissue to obtain protein for Western blots:
http://biologicalworld.com/lyseprimarycells.htm

Tnank you very much
i will try that

-Zhenya-