tissue "fixation" - (Nov/12/2008 )

Hey,

We are currently wondering how one could kill all cells in a certain tissue (e.g. skin biopsy) but retain the tissue's structure and functionality (and the cells therein) in the original state. Meaning too much crosslinking shouldn't take place. Azide and sublytic concentrations of Triton-X would lead to cell alterations (necrosis/apotosis) we don't want. I don't know about low PFA concentrations for a short incubation time.
Any ideas?

Ta.
Holger

Freeze them, then cryosection?