Immunohistochemistry of anti-ki-67 on paraformaldehyde-fixed mouse frozen tissue - (Nov/15/2007 )

Hello, I want to perform IHC on mouse (uterus) frozen tissu fixed with paraformaldehyde with the proliferation marker Ki-67.
First, what is the best Ki-67 antibody (company and #clone) and is there a special protocol which could give the best results (only the major points to consider)?
Thank you very much
eldoc

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An antigen retrieval protocol might help with better detection.

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that would be triton x rather than boiling in citrate buffer

Ive tried them from dako, novocastra and vector and they are pretty foolproof - acetone might be a better fix than Pfa

Ki67 is pretty easy - i use it and beta catenin when i'm teaching people immuno for the first time

dom

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