What are the benefits to extract DNA from tissues such as liver, heart and kidne - (Apr/12/2008 )
Hi everyone, this few days I was thinking the benefits to extract DNA from tissues (liver, heart, kidney) rather than other part of our body such as skin, blood etc.
Can anyone tell me the benefits ? Thanks for answering my stupid question ....
Can anyone tell me the benefits ? Thanks for answering my stupid question ....
I think it depends on your planned use. If you are just after DNA for genotyping or fingerprinting, skin, blood etc are fine. But if you're after information about gene expression, you might need tissue extracts, that is for mRNA extraction.
Can anyone tell me the benefits ? Thanks for answering my stupid question ....
I think it depends on your planned use. If you are just after DNA for genotyping or fingerprinting, skin, blood etc are fine. But if you're after information about gene expression, you might need tissue extracts, that is for mRNA extraction.
Higher yield.
You might want to use different tissues when you're looking for a chimeric mutation....
Some tissues are tricky to work with because of inhibitors and contaminants and so it's better to avoid their use (if possible).
I can't think about an example in human/animal biology, but some plant tissues are rich in polyphenols and other organic compounds and that makes it difficult to extract a good quality DNA/RNA.
following the idea of swanny, DNA methylation patterns change across tissues, in plants and animals