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Total protein extraction from Leaf tissue - (Apr/17/2007 )

Hi all,
I am trying to extract total protein from leaf tissue from a tree species.
Can anybody please suggest a good protocol.
I have to use the total protein for western blot to check my target protein.

Thanks in advance
Ranadheer

-ranadheer-

QUOTE (ranadheer @ Apr 17 2007, 06:16 AM)
Hi all,
I am trying to extract total protein from leaf tissue from a tree species.
Can anybody please suggest a good protocol.
I have to use the total protein for western blot to check my target protein.

Thanks in advance
Ranadheer


Here is our discussion for a good protocol for plant-protein extraction...

-ms-olli-