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SiRNA Related Discussions
  1. siRNA modification to make it more hydrophobic - (reply: 1)
  2. microRNA in situ hybridization - label probe 5' or 3'? (reply: 1)
  3. Validation of MicroRNA array results - (reply: 1)
  4. siRNA designation for different genes - (reply: 1)
  5. Double SiRNA trasfection - (reply: 1)
  6. siRNA into RAW264.7 cells? - any ideas (reply: 4)
  7. Sirna concentration - (reply: 1)
  8. suggested siRNA concentrations - (reply: 1)
  9. Invitrogen's Negative control siRNA - (reply: 3)
  10. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
  11. Dissolve siRNA in non-RNase free buffer - (reply: 1)
  12. CD4 siRNA - (reply: 1)
  13. Optimization of siRNA experiments - (reply: 1)
  14. Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
  15. Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES? (reply: 3)
  16. analyis of microRNA array data - (reply: 4)
  17. siRNA toxicity in MCF7 cells - why are they sick????? (reply: 1)
  18. siRNA in organotypic cultures - siRNA-mediated gene silencing in vitro and in vivo (reply: 6)
  19. Possible transfect siRNA using Calcium Phosphete? - siRNA transfection (reply: 2)
  20. siRNA vs. miRNA - (reply: 5)
  21. HUVEC siRNA transfection help please - I need suggestions on what else to try! (reply: 3)
  22. Using IRES in microRNA expression vector - (reply: 4)
  23. test the knockdown effficiency with siRNA before transfection with lentivirus? - (reply: 1)
  24. siRNA transfection in MLE12 cells - (reply: 3)
  25. siRNA for retinal pigment epithelial cells - (reply: 1)
  26. Scaling up siRNA transfection - (reply: 1)
  27. Co-Transfection siRNA and targeting construct - (reply: 3)
  28. siRNA transfection: DNA conc optimization problem - (reply: 1)
  29. Which Kit should I use for microRNA microarray? - microRNA Microarray (reply: 3)
  30. siRNA transfection in endothelial cells - (reply: 3)
  31. siRNA plus drugs - weird cell morphology (reply: 3)
  32. double siRNA knockdown? - procedure for simultaneous silencing (reply: 17)
  33. transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
  34. need help on microRNA real-time PCR - (reply: 6)
  35. Should siRNA sequence be 100% complementary? - (reply: 4)
  36. siRNA transfection - (reply: 4)
  37. easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
  38. siRNA transfection in primary hepatocytes - (reply: 1)
  39. validated siRNA not working plz help - (reply: 4)
  40. Transfection of siRNA in suspension cells - (reply: 6)
  41. gene duplication - affected by siRNA? (reply: 1)
  42. cholesterol conjugated siRNA - (reply: 2)
  43. Has anybody ever used Dharmacon ON-TARGETplus SMARTpool siRNA? - (reply: 6)
  44. siRNA in primary lymphocyte B - Which reagent do you recomend? (reply: 2)
  45. microRNA and FFPE - (reply: 1)
  46. is microRNA degraded with target? - is there any known pathway for miR degradation? (reply: 2)
  47. good reagents for transfection of siRNA in Caco-2 - (reply: 1)
  48. Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
  49. siRNA "knock-out"/transfection "knock-in" - (reply: 10)
  50. (newbie) what makes siRNA and miRNA different - (reply: 2)
  51. siRNA knock down did not work - protein expression knock down by siRNA (reply: 4)
  52. General List of Off Target Effects of siRNA - (reply: 1)
  53. siRNA transfection of TWO targets simultaneously - Any experience/advice? (reply: 1)
  54. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  55. knocking down mature miRNA with siRNA - is it possible to knockdown miRNA with siRNA? (reply: 4)
  56. siRNA positive control - (reply: 3)
  57. detect siRNA derived from shRNA construct - (reply: 5)
  58. SiRNA in MIN6 cells, nucleofection - (reply: 1)
  59. how to detect double strand siRNA - (reply: 2)
  60. siRNA stable cell lines - (reply: 6)
  61. SiRNA Transfection in 60mm plates - (reply: 2)
  62. siRNA transfection - (reply: 5)
  63. siRNA causing no protein knockdown - (reply: 1)
  64. siRNA transfection - (reply: 3)
  65. validated siRNA doesn't work - (reply: 6)
  66. sirna half life - time of effect (reply: 2)
  67. DNA contamination in microRNA sample - How to remove the genomic DNA from microRNA sample? (reply: 5)
  68. siRNA cost - siRNA cost (reply: 4)
  69. siRNA + DNA transfection - (reply: 3)
  70. mRNA expression low/no effect of the siRNA - (reply: 1)
  71. siRNA: in vivo delivery - (reply: 11)
  72. Cytoplasmic granulation after siRNA transfection - (reply: 3)
  73. overexpression of a microRNA cluster - (reply: 3)
  74. how to evaluate the siRNA silence efficiency? - western or rtPCR (reply: 6)
  75. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
  76. shRNA versus siRNA - (reply: 6)
  77. GFP siRNA transfection - readout - (reply: 5)
  78. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
  79. siRNA protocol in floating cells - (reply: 1)
  80. siRNA transfection problem - (reply: 4)
  81. U6, H1 siRNA - (reply: 1)
  82. siRNA transfection reagent - (reply: 9)
  83. siRNA and Hybond XL - any one use them? (reply: 1)
  84. siRNA: how long will knock-down last - (reply: 6)
  85. miRNA targets in comparison to siRNA targets - (reply: 2)
  86. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
  87. Basic question about siRNA, shRNA..etc - What's the difference? (reply: 5)
  88. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
  89. miRNA promoter ? - how to discover MicroRNA promoter element (reply: 1)
  90. Sources for vector-based siRNA against kinases? - (reply: 1)
  91. About the conc. use of the siRNA - (reply: 4)
  92. is there any fast screening after siRNA knockdown of protein? - (reply: 3)
  93. siRNA and mices - What is the best way to in vivo siRNA transfer (reply: 6)
  94. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
  95. Knocking down miRNA using siRNA - (reply: 5)
  96. Low transfection of siRNA into HaCaT cells. Please help - (reply: 1)
  97. shRNA/siRNA which loop sequence? - (reply: 2)
  98. shRNA/siRNA stem length 19, 20, or 21? - (reply: 3)
  99. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  100. siRNA - sh and si RNA RJNAi (reply: 6)
  101. siRNA in vivo use - (reply: 8)
  102. Two simple questions about microRNA's name - (reply: 2)
  103. How to increase RNAi efficiency by siRNA transfection? - (reply: 4)
  104. siRNA delivery in non-dividing cells - (reply: 8)
  105. How to trace siRNA in cells - (reply: 4)
  106. Time course for siRNA inhibition - When is the peak of inhibition after transfection ? (reply: 11)
  107. Exiqon (miRCURY) microRNA microarray - (reply: 3)
  108. help with choosing model system for optimization of transfection of siRNA - and best deal for positive control? (reply: 5)
  109. shelf life of siRNA - (reply: 1)
  110. siRNA failure - Knock down failure with oligofectamine (reply: 5)
  111. Confirming SiRNA Knockdown - (reply: 6)
  112. siRNA duplex length, 27mer? - anyone tested that 27mer is better? (reply: 1)
  113. Difference between SiRNA and shRNA - (reply: 2)
  114. siRNA transfection of MIN6 cells - (reply: 2)
  115. siRNA extraction from plant material - (reply: 5)
  116. siRNA purification - (reply: 2)
  117. siRNA Cloning method - (reply: 2)
  118. Effect of scrambled siRNA on gene expression - (reply: 5)
  119. siRNA delivery into human primary macrophages - (reply: 3)
  120. Puzzle about methylation on plant microRNA - (reply: 1)
  121. how to get poly(A) tailed microRNA - (reply: 1)
  122. Tool for making scrambled control siRNA - (reply: 1)
  123. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
  124. siRNA in non-adherent cell lines? - Tips please (reply: 4)
  125. How to design siRNAs for RNAi - (reply: 2)
  126. How long DNA and siRNA stay in transfected cells - (reply: 2)
  127. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
  128. How to know siRNA transfection efficiency? - (reply: 4)
  129. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
  130. about siRNA and shRNA - RNAi Vector (reply: 2)
  131. siRNA in vitro transcription-Ambion kit? - (reply: 3)
  132. siRNA to shRNA problem - (reply: 7)
  133. How to extract siRNA from primordial leaf - (reply: 1)
  134. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
  135. Basic questions about siRNA - (reply: 6)
  136. siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)
  137. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  138. siRNA transfection reagent for primary cultured cells - (reply: 2)
  139. microRNA Cloning - Advice...anyone...please! (reply: 5)
  140. Control siRNA - (reply: 3)
  141. BLAST EST to identify possible microRNA? - (reply: 3)
  142. different siRNA for the same gene cause opposite effect - (reply: 6)
  143. nonspecific cellular responses - vector system vs synthetic siRNA - (reply: 1)
  144. siRNA Stable transfection in human fibroblast cells - (reply: 6)
  145. Quantitative gene knock down with siRNA - (reply: 1)
  146. Use of dual promoter for siRNA - (reply: 6)
  147. siRNA transfection in cardiomyocytes - siRNA transfection in neonatal cultured cardiomyocytes (reply: 1)
  148. siRNA kit - expensive (reply: 2)
  149. siRNA databases and shRNA libraries - (reply: 8)
  150. siRNA /shRNA agains EGFP - (reply: 4)
  151. siRNA control by Western blot - how do I get equal loading concentration? (reply: 7)
  152. siRNA transfetion in MEL (murine erythroleukemia) cell - (reply: 3)
  153. Identifying microRNA computationally? - (reply: 3)
  154. negative control siRNA - (reply: 13)
  155. how to convert RNA-based siRNA to DNA-based siRNA? - (reply: 1)
  156. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
  157. Designing siRNA expression vectors - (reply: 2)
  158. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
  159. siRNA overhangs - do they matter? (reply: 1)
  160. qPCR and siRNA - (reply: 2)
  161. siRNA finder - (reply: 3)
  162. Transfection of keratinocytes with siRNA - (reply: 2)
  163. siRNA in Drosophila S2 cells? - (reply: 4)
  164. siRNA transfection - (reply: 1)
  165. methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
  166. Pre-designed siRNAs - Using Dharmacon's and Ambions guranteed siRNAs (reply: 3)
  167. siRNA northern - (reply: 1)
  168. siRNA versus shRNA - (reply: 4)
  169. Scrambled siRNA anyone? - (reply: 2)
  170. siRNA transfection of primary lymphocytes - (reply: 1)
  171. siRNA purity - (reply: 4)
  172. siRNA to shRNA - (reply: 7)
  173. extraction of endogeneous mirna and sirna - (reply: 6)
  174. siRNA detection - Protocol for siRNA detection (reply: 3)
  175. Can I target non-coding sequence by siRNA - (reply: 6)
  176. Detection of microRNA - (reply: 5)
  177. micro-RNA prediction algorithm - MiRscan or any other code for miRNA prediction (reply: 3)
  178. SiRNA transfection in HEK293T - (reply: 1)
  179. Does siRNA transfection dependents on cell type - (reply: 2)
  180. siRNA transfection into neurons - (reply: 2)
  181. transfecting siRNA and a plasmid - (reply: 4)
  182. siRNA database - siRNA database (reply: 2)
  183. siRNA - augmentation instead of knockdown - siRNA - augmentation instead of knockdown (reply: 2)
  184. FITC-conjugated siRNA - (reply: 4)
  185. siRNA transfection into 3T3-L1 - (reply: 3)
  186. Genomic integration of siRNA expression cassette - Comparison of generated clones (reply: 3)
  187. microRNA alignment - (reply: 5)
  188. siRNA for Akt1 - use of commercial siRNA to down-regulate Akt (reply: 4)
  189. siRNA design: where it should be targeted? - (reply: 5)
  190. siRNA in embryo - (reply: 1)
  191. What is the different between microRNA and siRNA? - (reply: 4)
  192. How to transfect siRNA into the nucleus for transcriptional silencing? - Which transfection reagent? (reply: 9)
  193. How to transfect siRNA into suspension cell lines? - (reply: 3)
  194. siRNA detection - i'm totally lost (reply: 8)
  195. Antibiotics during siRNA transfection - (reply: 4)
  196. Different siRNA transfection agents - Have enyone tested them??? (reply: 44)
  197. chemical synthesized siRNA and rapidly dividing cells - (reply: 2)
  198. siRNA in vivo?? - (reply: 7)
  199. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
  200. several questions for siRNA target BLAST - (reply: 4)
  201. Is mRNA spliced with very small fragment treated with siRNA? - (reply: 3)
  202. siRNA transfection - siRNA transfection (reply: 2)
  203. concentrate siRNA - (reply: 1)
  204. siRNA chemical synthesis - which company is reliable? (reply: 2)
  205. stable transfection with siRNA expression vector - (reply: 6)
  206. RNA Interference - how to produce siRNA (reply: 10)
  207. siRNA target selection problem - (reply: 11)