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analyis of microRNA array data - (Sep/03/2008 )

Any one could please direct me to a post on this forum or share there expertise to analyze microRNA array data, we have used Exiqon plateform and has acquired flourescence intensities on Genepix scanner; Exiquon and mouse spke ins used as controls, it has only single channel (cy3) data.

Thanks in advance. laugh.gif

-honeyhappy-

QUOTE (honeyhappy @ Sep 3 2008, 01:00 AM)
Any one could please direct me to a post on this forum or share there expertise to analyze microRNA array data, we have used Exiqon plateform and has acquired flourescence intensities on Genepix scanner; Exiquon and mouse spke ins used as controls, it has only single channel (cy3) data.

Thanks in advance. laugh.gif


I'm too interrested in microarray analysis methods, if you have found a track, please feedback !
Thank you.

-MPK-

QUOTE (MPK @ Sep 21 2008, 01:56 PM)
QUOTE (honeyhappy @ Sep 3 2008, 01:00 AM)
Any one could please direct me to a post on this forum or share there expertise to analyze microRNA array data, we have used Exiqon plateform and has acquired flourescence intensities on Genepix scanner; Exiquon and mouse spke ins used as controls, it has only single channel (cy3) data.

Thanks in advance. laugh.gif


I'm too interrested in microarray analysis methods, if you have found a track, please feedback !
Thank you.


I have analyzed the data using Gene spring which is quite user friendly.

-honeyhappy-

HI

After image analysis in genpix u can use even excel to analyse ur data .Hope ur aware of that

-gpbio-

Hi,

...do you have anyone in your group who is familar with R (aka r-project, a statistics program)?
In R you can import the package SAM (significant analysis of microarray data), which you can download from bioconductor.

Both r-project and SAM can be freely downloaded, SAM also supports a howto. Have a look.

CU

-xeroxed_yeti-