siRNA positive control - (Oct/02/2007 )

Hey researchers,
I've been looking for a good siRNA positive control. I started out an siRNA targeting Lamin A/C but it turns out Lamin A?C was not expressed in my HEK 293 cell line. I know GAPDH and actin are good controls as well. Does any happen to know where I can find the siRNA sequence for these two positive controls ( or any other that you happen to knwo is good)? I've tried searching at ambion but can not find the published sequence anywhere.

Thanks,
joe

-veryslowjoe-

Check whether you can find the sequence you wanted here http://www.rnainterference.org/Sequences.html

-pcrman-

Some people try to express gfp and then knock it down, as gfp siRNA sequence is quite well established and the readout is easy.

-scolix-

My favorite is siRNA against EGFP ( you can get it from IDT DNA). Better yet, the same siRNA agaist EGFPluc fusion gene (Clontech) for better quantitative purpose by measuring luc activity.

-genehunter-1-