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PCR--RT-PCR-and-Real-Time-PCR
91.
typical mRNA test with huge fold change? Any suggestions -
(reply: 1)
92.
PCR Amplification Issues and Primer dimer -
(reply: 4)
93.
Two consecutive ATG -
(reply: 2)
94.
How to make PCR analysis using the 22DDCT Method -
(reply: 1)
95.
qPCR data analysis from raw data -
(reply: 1)
96.
cDNA degradation within a week at -20? -
(reply: 2)
97.
Primer calculation for qPCR - final reaction -
(reply: 1)
98.
Sec. structure in primers -
(reply: 1)
99.
Differences in shape of melt curve -
(reply: 4)
100.
Amount of RNA for cDNA -
(reply: 4)
101.
Difference in melting temperature -
(reply: 5)
102.
Changing PCR recipe halfway -
(reply: 1)
103.
PCR master mix containing primers and Taq -
(reply: 4)
104.
Primer working with some cDNA samples and not other -
(reply: 2)
105.
no bands after pcr!!!! -
(reply: 4)
106.
Help with definitons for qPCR -
(reply: 1)
107.
Calibrator in qPCR -
(reply: 7)
108.
Experimental design for RT-PCR -
(reply: 8)
109.
Primer dilution -
(reply: 9)
110.
Calculation help -
(reply: 6)
111.
standards for qPCR and some other doubts -
(reply: 9)
112.
Plasma cortisol/corticosterone level analysis using qPCR -
(reply: 1)
113.
Technical Replicates vs Biological Replicates -
(reply: 1)
114.
pcr -
(reply: 1)
115.
Difference in synthesis -
(reply: 1)
116.
Standardizing cDNA concentration before doing PCR -
(reply: 4)
117.
Agarose Gel Electrophoresis -
(reply: 1)
118.
Volume of Primers -
(reply: 4)
119.
qPCR with different genes -
(reply: 5)
120.
PCR conditions unknown? -
(reply: 10)
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