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PCR--RT-PCR-and-Real-Time-PCR
61.
Strange Taqman probe negative fluorescence -
(reply: 2)
62.
qPCR std curve calculation issue if using PCR product as the std -
(reply: 1)
63.
qPCR , eDNA and standard curve -
(reply: 3)
64.
Thermal Cycler for Multiplex PCR -
(reply: 3)
65.
Problem of melt curve shape for pikoreal real time PCR -
(reply: 1)
66.
Primer sequences with M and Y - how to order? -
(reply: 2)
67.
QPCR with genomic DNA from blood -
(reply: 2)
68.
RT-PCR low efficiency -
(reply: 1)
69.
emulsion PCR -
(reply: 1)
70.
qPCR data analysis -
(reply: 2)
71.
Addition of DNase after RNA extraction -
(reply: 4)
72.
RT-PCR electrophorasis -
(reply: 1)
73.
PCR contamination -
(reply: 6)
74.
RNA agarose gel blurry fuzzy. RNase contaminaton? -
(reply: 2)
75.
cDNA serial dilution trouble in qPCR: help needed -
(reply: 2)
76.
High background fluorescence detected before starting PCR -
(reply: 3)
77.
Real-time pcr -
(reply: 1)
78.
Using qPCR machine for simple PCR, is it possible? -
(reply: 3)
79.
What could be the possible reasons for a RT-PCR experiment that was working fine -
(reply: 4)
80.
Combining standard error of normalised fold change values - Qbase method -
(reply: 2)
81.
What cycle is best for analysing data? -
(reply: 2)
82.
Mineral oil quality in PCR tubes -
(reply: 5)
83.
Internal control for absolute quantification -
(reply: 3)
84.
Diagnostic PCR troubleshooting -
(reply: 2)
85.
QPCR replicates issue -
(reply: 1)
86.
RT-PCR Kit's Validation -
(reply: 1)
87.
Rt pcr -
(reply: 4)
88.
Viral Quantification -
(reply: 1)
89.
Inhibition of PCR amplification of bacterial genomic DNA by RNA -
(reply: 1)
90.
Housekeeping genes - Cq values -
(reply: 4)
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