How to make PCR analysis using the 22DDCT Method - (Jun/17/2015 )

Hey,

I am new in the PCR analysis word, I would like to do the data analysis using  22DDCT Method.

I have CT values and the analysis from the reference and control gene...and here I am lost.

How I should  procedure to  to start my data  analysis?

Klaudek on Wed Jun 17 18:25:28 2015 said:

Hey,

 

I am new in the PCR analysis word, I would like to do the data analysis using  22DDCT Method.

I have CT values and the analysis from the reference and control gene...and here I am lost.

How I should  procedure to  to start my data  analysis?

 

 

This protocol will help you. It also gives a step by step example on how to calculate using the ddct method.

http://www.ncbi.nlm.nih.gov/pubmed/11846609