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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
542. Normalization to Standard Gene: what does this mean - (reply: 8)
543. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
544. How to Join two PCR products - (reply: 3)
545. Reference RNA for qPCR - (reply: 3)
546. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
547. What's wrong with my PCR - (reply: 5)
548. What does 'prevention of nontemplate-directed nucleotide addition' mean? - (reply: 3)
549. loss or results (ABI prism 7000) - (reply: 6)
550. Ct values - (reply: 3)
551. Problem with UPL real-time PCR - please help !!! - (reply: 1)
552. qRT-PCR data analysis - (reply: 2)
553. Cp, but no amplification - (reply: 2)
554. Strange RT to qPCR results with new kit - (reply: 5)
555. Low PCR product - (reply: 7)
556. SYBR vs FAM probe - (reply: 1)
557. Quantify gDNA in buccal swab - (reply: 2)
558. qPCR: band in gel, but super high Cq? - (reply: 10)
559. Melt curve analysis - (reply: 5)
560. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
561. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
562. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
563. wrong dntp concentration - (reply: 2)
564. Which one is better as reference gene base on these data - (reply: 3)
565. About the qPCRs and statistics - (reply: 2)
566. long fragment PCR - (reply: 2)
567. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
568. RT-qPCR normalization - (reply: 3)
569. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
570. I need to break this cycle of PCR issues - (reply: 13)