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PCR--RT-PCR-and-Real-Time-PCR
991.
Primers... -
(reply: 1)
992.
18s as endogenous control for Oligo dT RT - Can we use 18s ?
(reply: 2)
993.
excel and propagation of error, qpcr -
(reply: 1)
994.
Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els
(reply: 4)
995.
primer with higher melting temperature -
(reply: 2)
996.
Omitting Points in Standard Curve -
(reply: 2)
997.
Triage of miRNA targets by qPCR -
(reply: 1)
998.
Smallest reaction volume with Roche Lightcycler 480 96-well plate format -
(reply: 2)
999.
qPCR primer design - possible off-target
(reply: 1)
1000.
Primer dimer formation and real-time PCR -
(reply: 7)
1001.
5' race -
(reply: 2)
1002.
Identify PCR products -
(reply: 3)
1003.
Maximum size of overhangs in PCR -
(reply: 6)
1004.
water-droplets in my cycler !!! - experienced such thing ???
(reply: 9)
1005.
a second small peak to the right to my pricipal peak!!! -
(reply: 1)
1006.
qPCR virgin, What do I do first? - How to set up experiment
(reply: 1)
1007.
Colony PCR doesn't work anymore -
(reply: 5)
1008.
Question about TM and Annealing temperatures -
(reply: 4)
1009.
RT-PCR t-test, and ANOVA -
(reply: 1)
1010.
PCR basics - I'm new to all things lab and need some help!
(reply: 3)
1011.
the red dye in trizol? -
(reply: 2)
1012.
Is it acceptable that qpcr reaction doesn't reach plateau? -
(reply: 3)
1013.
What are exogeneous and endogeneous controls? -
(reply: 4)
1014.
quantitate wild type and mutant mRNA -
(reply: 1)
1015.
Can very low RNA concentration cause Ct bias..? -
(reply: 3)
1016.
smear in pcr +DNA polymerase mixture for long PCR -
(reply: 10)
1017.
iScript BioRad cDNA synthesis yield -
(reply: 1)
1018.
High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting
(reply: 5)
1019.
intron spanning primers for non model organisms -
(reply: 2)
1020.
Using DNA as a reference in qRT-PCR -
(reply: 1)
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