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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
421.
Perfect dilution curve, but double peak melting curve on SYBR qPCR -
(reply: 7)
422.
Real-time efficiency question -
(reply: 1)
423.
Selective removal of standards post qPCR Run -
(reply: 1)
424.
Primer concentration - stupid question -
(reply: 3)
425.
Primer Design, help i´m New -
(reply: 4)
426.
qPCR question (no results?) -
(reply: 1)
427.
Artificial RNA as normalizer (HK gene) -
(reply: 1)
428.
Sensitivity of RT-PCR and qPCR -
(reply: 4)
429.
Mixing two cDNA samples into one for realtime PCR -
(reply: 3)
430.
real time PCR trouble -
(reply: 3)
431.
removal of ethanol in PCR product purification -
(reply: 5)
432.
Crazy real time PCR curve -
(reply: 4)
433.
High fidelity PCR trouble shooting -
(reply: 2)
434.
Reproducible Non-Specific PCR Product -
(reply: 2)
435.
How to prepare serial dilutions of RNA or cDNA -
(reply: 3)
436.
Are these primer products good enough for qPCR? -
(reply: 3)
437.
mtDNA to nDNA Ratio gDNA - qPCR -
(reply: 1)
438.
Can't get PCR with large overhang primers to work -
(reply: 8)
439.
Primer Efficiency across runs -
(reply: 1)
440.
Question about pipet tips for PCR and rtPCR -
(reply: 4)
441.
Taqman Probe sequence problem -
(reply: 4)
442.
UNG in PCR -
(reply: 1)
443.
Cause of random samples failing PCR? -
(reply: 2)
444.
Template DNA for PCR- Concrentration or Volume?? -
(reply: 1)
445.
RNA extraction -
(reply: 3)
446.
Normalization of Ct of interest to a reference Ct -
(reply: 3)
447.
urgent: need help with qPCR statistics -
(reply: 2)
448.
A question for Reverse transcription experts -
(reply: 5)
449.
will mRNA from different cell line lead to loss of expected product in RT PCR? -
(reply: 2)
450.
PCR amplification with new restriction sites troubleshooting -
(reply: 2)
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