Difference in synthesis - (May/06/2015 )

Hello,

I am now in the process of ordering the primers. But i am confused as to which Synthesis scale should i consider. I am ordering from Sigma and they have 4 options: 0.025, 0.05, 0.2 and 1 micromole. I want to chose the cheapest option i.e. 0.025 micrmole. I just want to know the difference between all these 4 options and why is 0.025 micromole (very) cheaper as compared to 1 micrmole? What are the advantages of using 1 micrmole over 0.025 micromoles?

Thank you and awaiting your response.

25 nmole scale is usually far more of the oligo than necessary for most PCR or sequencing needs, unless you are planning on large scale experiments with hundreds of replicates.  It is cheaper because it can be done in high volume and is the "standard" process.  Other scales require special handling, are less common, and more costly (over the additional reagent cost).