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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
841.
qPCR Calc Help -
(reply: 6)
842.
ABI Prism 7700 software -
(reply: 1)
843.
Difficult sequences in PCR -
(reply: 2)
844.
qPCR result interpretation - when your 'relative' is 0?? -
(reply: 2)
845.
why is real time pcr curve like this? -
(reply: 3)
846.
Housekeeping gene for COS-1 cells -
(reply: 1)
847.
PCR, neg control & no. of cycles -
(reply: 2)
848.
How do I insert a DNA sequence into a multiple cloning site and add restriction -
(reply: 1)
849.
efficiency calculation from non-10 fold dilutions -
(reply: 1)
850.
whole plasmid pcr? -
(reply: 6)
851.
Proper storage of isolated white blood cell prior to RNA isolation -
(reply: 3)
852.
Genomic pseudogenes?!? -
(reply: 6)
853.
Strange amplification plot -
(reply: 4)
854.
Conventional PCR of different dilutions -
(reply: 1)
855.
primer resuspension -
(reply: 2)
856.
Principles of overlap PCR -
(reply: 7)
857.
Help designing primers with restriction sites -
(reply: 3)
858.
DNA sequence data came back with lots of wrong bases -
(reply: 5)
859.
Why primers shouldn't have Tm difference greater than 5°C? -
(reply: 5)
860.
Abnormal amplification curve -
(reply: 1)
861.
Problem with Semiquantitative PCR -
(reply: 4)
862.
false positive/cross reaction with TaqMan -
(reply: 1)
863.
Problem w/ Genotyping Cre Only -
(reply: 3)
864.
ROX reference dye for quantitative RT-PCR -
(reply: 1)
865.
PCR problem -
(reply: 9)
866.
Didint get any band during gel electrophoresis even though DNA was there -
(reply: 5)
867.
why PCR product smeared? -
(reply: 2)
868.
Tm calculation for primers with RE sites and overhangs -
(reply: 4)
869.
silincing box, no amplification -
(reply: 3)
870.
Comparative semiquantitative RT-PCR -
(reply: 2)
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