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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. no pcr amplification product - (reply: 9)
1712. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1713. less steep curve and high Ct value! - (reply: 2)
1714. Question about site directed mutagenesis - (reply: 1)
1715. Amplfication of fungal DNA - (reply: 8)
1716. PCR problem - (reply: 4)
1717. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1718. differently behavioring replicates in real-time PCR - (reply: 1)
1719. Multiplex PCR - (reply: 1)
1720. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1721. optimal template dna concentrations - (reply: 1)
1722. reconstitution of primers - (reply: 2)
1723. Mysterious PCR Contamination - (reply: 13)
1724. RT-PCR Help - Protocol provided (reply: 4)
1725. Endogenous control in bacterial RT-qPCR - (reply: 4)
1726. Mutagenesis PCR and undesired amplification - (reply: 2)
1727. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1728. primer dimer - (reply: 2)
1729. Genotyping conditioning-where to start? - (reply: 1)
1730. Marker migrates slower depending where is loaded - (reply: 2)
1731. PCR on degraded templates - (reply: 2)
1732. PCR help needed - (reply: 3)
1733. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1734. Tm difference - (reply: 2)
1735. Genotyping - PCR not working (reply: 6)
1736. RT qPCR Newbie, Weird Results - (reply: 1)
1737. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1738. light bands, dark smear, dark dimers - (reply: 2)
1739. PCR, followed by sequencing... - why ?? (reply: 3)
1740. qPCR and varying cDNA concentrations - (reply: 1)