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1921. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
1922. maximum amplicon size - 550bp too long??? (reply: 5)
1923. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
1924. SYBR Green miRNA analysis - (reply: 1)
1925. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
1926. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
1927. Can DEPC-water interfere QPCR reaction? - (reply: 4)
1928. Melting Curve Analysis Difficulties - (reply: 7)
1929. SYBR and TaqMan - (reply: 6)
1930. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
1931. New Technology in Real Time PCR - latest technologies (reply: 2)
1932. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
1933. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
1934. PCR efficiencies - (reply: 4)
1935. Optimizing multiplex RT-qPCR - (reply: 3)
1936. difference between hotstart and taq polymerase - (reply: 7)
1922. maximum amplicon size - 550bp too long??? (reply: 5)
1923. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
1924. SYBR Green miRNA analysis - (reply: 1)
1925. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
1926. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
1927. Can DEPC-water interfere QPCR reaction? - (reply: 4)
1928. Melting Curve Analysis Difficulties - (reply: 7)
1929. SYBR and TaqMan - (reply: 6)
1930. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
1931. New Technology in Real Time PCR - latest technologies (reply: 2)
1932. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
1933. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
1934. PCR efficiencies - (reply: 4)
1935. Optimizing multiplex RT-qPCR - (reply: 3)
1936. difference between hotstart and taq polymerase - (reply: 7)