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1891. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1892. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1893. Dnase digestion and PCR - (reply: 4)
1894. Understanding qPCR and gene expression changes - (reply: 10)
1895. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1896. Reverse Transcription - (reply: 3)
1897. Plasmid as standard - Plasmid calculation (reply: 6)
1898. what Ct values are considered ideal? too high? - (reply: 1)
1899. Need an UV260 RT-PCR instrument - (reply: 1)
1900. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1901. PCR produces products of wrong size - (reply: 4)
1902. PCR with pfu and degenerate primers (?) - (reply: 7)
1903. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1904. Serious issues with RT-PCR - (reply: 7)
1905. relative quantitation problem plz help - (reply: 4)
1906. No beta actin results at all??? - (reply: 1)
1907. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1908. dNTP question - (reply: 3)
1909. Taqman rtPCR primer and probe design - (reply: 3)
1910. Help with Real Time PCR Well To Well Variation - (reply: 10)
1911. dna for real time - (reply: 3)
1912. Mycobacterial housekeeping genes - (reply: 1)
1913. Expression comparision using Cp values - (reply: 1)
1914. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1915. Which to use for standard curve? - (reply: 2)
1916. How do you calibrate between runs? - (reply: 3)
1917. Houskeeping Gene not steady - (reply: 7)
1918. real time PCR - (reply: 3)
1919. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1920. Plateau Phase - Taqman not reaching plateau (reply: 1)
1892. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1893. Dnase digestion and PCR - (reply: 4)
1894. Understanding qPCR and gene expression changes - (reply: 10)
1895. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1896. Reverse Transcription - (reply: 3)
1897. Plasmid as standard - Plasmid calculation (reply: 6)
1898. what Ct values are considered ideal? too high? - (reply: 1)
1899. Need an UV260 RT-PCR instrument - (reply: 1)
1900. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1901. PCR produces products of wrong size - (reply: 4)
1902. PCR with pfu and degenerate primers (?) - (reply: 7)
1903. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1904. Serious issues with RT-PCR - (reply: 7)
1905. relative quantitation problem plz help - (reply: 4)
1906. No beta actin results at all??? - (reply: 1)
1907. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1908. dNTP question - (reply: 3)
1909. Taqman rtPCR primer and probe design - (reply: 3)
1910. Help with Real Time PCR Well To Well Variation - (reply: 10)
1911. dna for real time - (reply: 3)
1912. Mycobacterial housekeeping genes - (reply: 1)
1913. Expression comparision using Cp values - (reply: 1)
1914. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1915. Which to use for standard curve? - (reply: 2)
1916. How do you calibrate between runs? - (reply: 3)
1917. Houskeeping Gene not steady - (reply: 7)
1918. real time PCR - (reply: 3)
1919. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1920. Plateau Phase - Taqman not reaching plateau (reply: 1)