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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
1021.
minimum replicate for qpcr? -
(reply: 15)
1022.
Primer design -
(reply: 2)
1023.
Troubleshoot PCR, Product band missing, internal control is present -
(reply: 1)
1024.
qPCR amplification interference fixed with freezing -
(reply: 6)
1025.
Suggest me a mastermix for conventinal PCR... -
(reply: 11)
1026.
reaction efficiency -
(reply: 4)
1027.
software for quantifing PCR product band -
(reply: 1)
1028.
Thermal cycler -
(reply: 2)
1029.
Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve?
(reply: 3)
1030.
qPCR volume - Problem with volume difference and results
(reply: 1)
1031.
RT-PCR -
(reply: 9)
1032.
Best way to mix qPCR master mix and high ct deviation questions -
(reply: 3)
1033.
help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand
(reply: 4)
1034.
cDNA synthesis -
(reply: 6)
1035.
Will I need interplate calibration for my RT-PCR experiment? -
(reply: 3)
1036.
Primer seq -
(reply: 2)
1037.
Selection of housekeeping gene in Mycobacterium -
(reply: 1)
1038.
Do I need to pool the base line samples together -
(reply: 2)
1039.
PCR product size -
(reply: 6)
1040.
amplification plot raises to early - What is the explanation for this problem?
(reply: 1)
1041.
Few basic questions about RNA -
(reply: 3)
1042.
oligo dt and random primer -
(reply: 3)
1043.
Ct's of goi higher and lower than ct's hkg -
(reply: 1)
1044.
Effect of collagenase on qpcr results -
(reply: 1)
1045.
RNA Isolation -
(reply: 3)
1046.
mRNA Search for RT-PCR (U to T) -
(reply: 2)
1047.
First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers
(reply: 1)
1048.
PCR triplicates versus one reaction - differences?
(reply: 17)
1049.
cloning of race products -
(reply: 1)
1050.
Single or double-stranded cDNA? -
(reply: 2)
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