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PCR--RT-PCR-and-Real-Time-PCR
1051.
Comparing SYBR and probe-based Ct values - Is it valid?
(reply: 2)
1052.
qPCR inhibition -
(reply: 1)
1053.
what reference gene will you use to compare neuroblastoma, glioblastoma? -
(reply: 1)
1054.
Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR -
(reply: 2)
1055.
Do low binding 0.2ml PCR tubes exist? -
(reply: 3)
1056.
Primers and self and hetero dimers -
(reply: 1)
1057.
Really weird amplification curves -
(reply: 1)
1058.
Primers for qPCR -
(reply: 3)
1059.
copy number calculation from cDNA -
(reply: 1)
1060.
Placing dNTP in 64 C waterbath - Would this ruin the solution?
(reply: 3)
1061.
RT-PCR primer -
(reply: 3)
1062.
CT value>25 with GAPDH in 50-fold dilution! -
(reply: 3)
1063.
PCR problem - basic PCR for plasmid amplification
(reply: 2)
1064.
can RNA amplify in two-step qPCR? -
(reply: 2)
1065.
Weird bands in standard PCR of gDNA and cDNA -
(reply: 2)
1066.
Nested LA PCR - Any success?
(reply: 3)
1067.
Unpredictablity of PCR product -
(reply: 5)
1068.
total cDNA amplification by PCR -
(reply: 2)
1069.
Detection limit of a conventional PCR -
(reply: 2)
1070.
Dissolved Primer pellet in pure ethanol instead of water -
(reply: 2)
1071.
RNAi showing upregulation via QPCR - Help needed
(reply: 3)
1072.
DNA detection limit of the PCR - Calculating detection limits
(reply: 4)
1073.
cDNA synthesis -
(reply: 3)
1074.
2 rounds PCR got problem -
(reply: 2)
1075.
PCR help minus strand - Primer design
(reply: 1)
1076.
cDNA contamination, pls help! -
(reply: 1)
1077.
Smear in long distance PCR -
(reply: 39)
1078.
TP-PCR or three primer PCR - TP-PCR or three primer PCR
(reply: 9)
1079.
Help - I m trying to get amplification for a new gene with new set of primers
(reply: 2)
1080.
can contaminating gDNA explain this? -
(reply: 1)
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