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1. different Ct values for RNaseP gene in human genomic DNA - (reply: 1)
2. Erratic Cq values from Plasmids - (reply: 5)
3. ABI 7900HT big alarm - (reply: 1)
4. Light genotyping PCR bands - (reply: 4)
5. What is the relation between amplicon size and melting temp? - (reply: 2)
6. Viral isolation kit QIA buffer substitute - (reply: 3)
7. Efficiency curve of primers - (reply: 1)
8. How do determine the reference gene for SMN 2 - (reply: 1)
9. Dengue Virus Serotyping - (reply: 1)
10. gain optimization closest match selected - (reply: 1)
11. Low amount of RNA and cDNa for qPCR - (reply: 1)
12. An odd situation: rfu values & scoring of AFLP fragments in clonally propaga - (reply: 2)
13. How to publish real time PCR results? - (reply: 2)
14. issue with PCR - (reply: 1)
15. RNA degradation from cells embedded in gels - (reply: 4)
16. ITS gene PCR Smears - (reply: 6)
17. Trouble with PCR after cloning - (reply: 1)
18. Problem with cDNA synthesis - (reply: 2)
19. real-time PCR machine error - (reply: 1)
20. what may have gone wrong? - (reply: 21)
21. bands are not discrete in PCR, why? - (reply: 1)
22. Verifying positive during PCR amplification - (reply: 1)
23. how to predict amplicon size? - (reply: 1)
24. Mastermix expiry dates? - (reply: 2)
25. Optimization of real time-PCR - where should I start from? - (reply: 2)
26. Weird PCR results - (reply: 2)
27. Primer design (Real time PCR) - (reply: 2)
28. Exact amplification in negative control??? - (reply: 1)
29. PCR is inconsistent - (reply: 4)
30. Specific primer problem - (reply: 2)
2. Erratic Cq values from Plasmids - (reply: 5)
3. ABI 7900HT big alarm - (reply: 1)
4. Light genotyping PCR bands - (reply: 4)
5. What is the relation between amplicon size and melting temp? - (reply: 2)
6. Viral isolation kit QIA buffer substitute - (reply: 3)
7. Efficiency curve of primers - (reply: 1)
8. How do determine the reference gene for SMN 2 - (reply: 1)
9. Dengue Virus Serotyping - (reply: 1)
10. gain optimization closest match selected - (reply: 1)
11. Low amount of RNA and cDNa for qPCR - (reply: 1)
12. An odd situation: rfu values & scoring of AFLP fragments in clonally propaga - (reply: 2)
13. How to publish real time PCR results? - (reply: 2)
14. issue with PCR - (reply: 1)
15. RNA degradation from cells embedded in gels - (reply: 4)
16. ITS gene PCR Smears - (reply: 6)
17. Trouble with PCR after cloning - (reply: 1)
18. Problem with cDNA synthesis - (reply: 2)
19. real-time PCR machine error - (reply: 1)
20. what may have gone wrong? - (reply: 21)
21. bands are not discrete in PCR, why? - (reply: 1)
22. Verifying positive during PCR amplification - (reply: 1)
23. how to predict amplicon size? - (reply: 1)
24. Mastermix expiry dates? - (reply: 2)
25. Optimization of real time-PCR - where should I start from? - (reply: 2)
26. Weird PCR results - (reply: 2)
27. Primer design (Real time PCR) - (reply: 2)
28. Exact amplification in negative control??? - (reply: 1)
29. PCR is inconsistent - (reply: 4)
30. Specific primer problem - (reply: 2)