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SDS-PAGE-and-Western-Blotting
361.
Determining pI of a protein -
(reply: 3)
362.
Non-Reduced Western Blot -
(reply: 5)
363.
Incomplete transfer -
(reply: 5)
364.
can coommassie stained gel be western blot -
(reply: 5)
365.
Wet transfer: western blot -
(reply: 3)
366.
Reprobing a PVDF membrane which has gone dry -
(reply: 1)
367.
Non-specific bands and fuzzy bands -
(reply: 1)
368.
protein showing up around 50 kda rather than 20 kda -
(reply: 2)
369.
Help for western blot wet transfer blurry bands -
(reply: 2)
370.
anti-BRCA1 antibody specificity issues -
(reply: 2)
371.
Concentrating a diluted protein and protein determination -
(reply: 4)
372.
Blurry bands ... -
(reply: 12)
373.
Dot blotting problems -
(reply: 3)
374.
2 primary antibodies, both binding actin...they shouldn't be :( -
(reply: 3)
375.
Uneven lane widths during western blots -
(reply: 10)
376.
Using human serum as first antibody in Western/ELISA -
(reply: 3)
377.
Strip Western Blotting Troubleshooting -
(reply: 2)
378.
Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? -
(reply: 3)
379.
Phospho-ATF-2 coming up different sizes -
(reply: 1)
380.
Fold-changes different when quantifying same protein via IF and Western -
(reply: 1)
381.
A possible protocol for western blotting troubleshooting? -
(reply: 1)
382.
Vertical Vs horizontal electrophoresis -
(reply: 3)
383.
Reprobing with the same antibody -
(reply: 3)
384.
SDS-PAGE problem with staining yellow background -
(reply: 3)
385.
SDS-PAGE for cell culture medium - sample preparation -
(reply: 1)
386.
Western blot narrow bands -
(reply: 1)
387.
Grainy image and no signal on Western using ChemiDoc -
(reply: 6)
388.
Western Blot Antibody Epitope -
(reply: 1)
389.
Western blot band anaylisis -
(reply: 3)
390.
protein aggregation (?) in gelatin zymography -
(reply: 1)
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