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841. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
842. Western with conjugated antibody? - (reply: 2)
843. Protein bands on the bottom of the gel - (reply: 5)
844. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
845. Tube gel IEF problems - Polymerization... (reply: 2)
846. Cannot detect GFP using GFP antibody - (reply: 10)
847. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
848. Acrylamide/bis stock expiration? - (reply: 2)
849. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
850. Incubation with washing buffer only? - (reply: 4)
851. Ponceau Re-staining - (reply: 1)
852. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
853. color of 5 times SDS loading dye - (reply: 1)
854. Protein concentration and - (reply: 3)
855. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
856. Multiple antibodies give no signal - (reply: 10)
857. Problem with protein samples - No bands detected at all on gel (reply: 2)
858. using cell lysate to run native gel? - (reply: 1)
859. Bradford Query - (reply: 5)
860. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands？ (reply: 9)
861. About GFP- tagged proteins in western blot - (reply: 1)
862. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
863. PVP-40 for blocking? - Instead of milk (reply: 2)
864. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
865. How to pase my Co-IP over the weekend? - (reply: 1)
866. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
867. Protein Sample Preparation - Western Bloting (reply: 1)
868. No signal at all - (reply: 16)
869. Centrifuge after lysing - (reply: 5)
870. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
842. Western with conjugated antibody? - (reply: 2)
843. Protein bands on the bottom of the gel - (reply: 5)
844. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
845. Tube gel IEF problems - Polymerization... (reply: 2)
846. Cannot detect GFP using GFP antibody - (reply: 10)
847. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
848. Acrylamide/bis stock expiration? - (reply: 2)
849. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
850. Incubation with washing buffer only? - (reply: 4)
851. Ponceau Re-staining - (reply: 1)
852. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
853. color of 5 times SDS loading dye - (reply: 1)
854. Protein concentration and - (reply: 3)
855. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
856. Multiple antibodies give no signal - (reply: 10)
857. Problem with protein samples - No bands detected at all on gel (reply: 2)
858. using cell lysate to run native gel? - (reply: 1)
859. Bradford Query - (reply: 5)
860. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands？ (reply: 9)
861. About GFP- tagged proteins in western blot - (reply: 1)
862. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
863. PVP-40 for blocking? - Instead of milk (reply: 2)
864. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
865. How to pase my Co-IP over the weekend? - (reply: 1)
866. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
867. Protein Sample Preparation - Western Bloting (reply: 1)
868. No signal at all - (reply: 16)
869. Centrifuge after lysing - (reply: 5)
870. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)